Hotspot

trnR-ACG-rrn5

Genome · 162133-162380 · 248 bp

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Pi 0.15

MarkerSeek score 74.30

Primer available yes

Label trnR-ACG-rrn5

Feature Metadata

label_name: trnR-ACG-rrn5
feature_type: igs
region: Genome
length_bp: 248
variable_sites: 128
pi: 0.15
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn5_p1	CCGTTGTTCCTCAGTAGCTC	CAGCTGAGGCATCCTAACAG	55.0	55.0	58.0	58.0	1306-1310 bp	1.0	91.4
trnR-ACG-rrn5_p2	CCGTTGTTCCTCAGTAGCTC	CCTTGTTCCTACATGACCCG	55.0	55.0	58.0	58.0	1271-1275 bp	1.0	91.1
trnR-ACG-rrn5_p3	CCGTTGTTCCTCAGTAGCTC	TGTCCCCTCCAGTCAAGAAT	55.0	50.0	58.0	58.0	1111-1115 bp	1.0	91.1
trnR-ACG-rrn5_p4	TAGCGCATCAAAGATGCAGT	CCTTGTTCCTACATGACCCG	45.0	55.0	58.0	58.0	1048-1052 bp	1.0	90.9
trnR-ACG-rrn5_p5	CCGTTGTTCCTCAGTAGCTC	TGCCTCTCCAAGGGATGTAA	55.0	50.0	58.0	58.0	1149-1151 bp	0.285714	51.3