Hotspot

trnN-GUU-trnR-ACG

Genome · 161546-162058 · 513 bp

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Pi 0.144
MarkerSeek score 75.90
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
Genome
length_bp
513
variable_sites
253
pi
0.144
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CTCGACGCCAGGATGTTAAA 55.0 50.0 58.0 58.0 924-928 bp 1.0 91.9
trnN-GUU-trnR-ACG_p2 CAGTACCCTCGTTGACTTCG CTCGACGCCAGGATGTTAAA 55.0 50.0 58.0 58.0 1221-1225 bp 1.0 91.8
trnN-GUU-trnR-ACG_p3 CAGTACCCTCGTTGACTTCG CCTTGTTCCTACATGACCCG 55.0 55.0 58.0 58.0 1568-1572 bp 1.0 91.6
trnN-GUU-trnR-ACG_p4 CCGTTGTTCCTCAGTAGCTC CCTTGTTCCTACATGACCCG 55.0 55.0 58.0 58.0 1271-1275 bp 1.0 91.1
trnN-GUU-trnR-ACG_p5 CCGTTGTTCCTCAGTAGCTC TGCCTCTCCAAGGGATGTAA 55.0 50.0 58.0 58.0 1149-1151 bp 0.285714 51.3