Hotspot

rps15-trnN-GUU

Genome · 155487-161473 · 5987 bp

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Pi 0.155
MarkerSeek score 76.30
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
Genome
length_bp
5987
variable_sites
2713
pi
0.155
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 TCCCGCAAAAATTCTGGACA AAGACAACCAGAGACGGGTA 45.0 50.0 58.0 58.0 1760-1871 bp 0.714286 80.4
rps15-trnN-GUU_p2 CGACTCGACAAGACAACGAT TCAGTGATTGGAGGTGGACT 50.0 50.0 58.0 58.0 1624-1731 bp 0.571429 73.3
rps15-trnN-GUU_p3 ATACAAAGGAGAGCCCCTGT TCAGTGATTGGAGGTGGACT 50.0 50.0 58.0 58.0 1396-1412 bp 0.428571 66.4
rps15-trnN-GUU_p4 TCCCGCAAAAATTCTGGACA CGATCTACAATAGGCCGCTC 45.0 55.0 58.0 58.0 1694-1801 bp 0.428571 65.7
rps15-trnN-GUU_p5 TCCCGCAAAAATTCTGGACA TCAGTGATTGGAGGTGGACT 45.0 50.0 58.0 58.0 1872-1979 bp 0.428571 65.2