Hotspot

trnN-GUU-rps15

Genome · 120672-126658 · 5987 bp

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Pi 0.146

MarkerSeek score 76.20

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: Genome
length_bp: 5987
variable_sites: 2585
pi: 0.146
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	GGCAGCGAATCGAACAAAAA	TACCCGTCTCTGGTTGTCTT	45.0	50.0	58.0	58.0	538-1983 bp	0.857143	85.9
trnN-GUU-rps15_p2	TATTCGACTGGAATGGCAGC	TACCCGTCTCTGGTTGTCTT	50.0	50.0	58.0	58.0	552-1997 bp	0.857143	85.9
trnN-GUU-rps15_p3	TCGTCCGCTTGACAATACTG	TTTTTGTTCGATTCGCTGCC	50.0	45.0	58.0	58.0	869-1155 bp	0.571429	74.4
trnN-GUU-rps15_p4	TCGTCCGCTTGACAATACTG	GAGCGGCCTATTGTAGATCG	50.0	55.0	58.0	58.0	357-1789 bp	0.571429	73.7
trnN-GUU-rps15_p5	TCGTCCGCTTGACAATACTG	AGTCCACCTCCAATCACTGA	50.0	50.0	58.0	58.0	179-1611 bp	0.571429	71.1