Hotspot

rps16-rrn16

Genome · 109644-112452 · 2809 bp

Back to Vaccinium

Pi 0.106

MarkerSeek score 78.60

Primer available yes

Label rps16-rrn16

Feature Metadata

label_name: rps16-rrn16
feature_type: igs
region: Genome
length_bp: 2809
variable_sites: 811
pi: 0.106
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-rrn16_p1	TGCTCAACCTACAGAAACGG	CCAGGATCGAACTCTCCATG	50.0	55.0	57.8	57.5	1601-2980 bp	0.857143	79.3
rps16-rrn16_p2	GCTCAACCTACAGAAACGGT	CCAGGATCGAACTCTCCATG	50.0	55.0	57.8	57.5	1600-2979 bp	0.857143	79.3
rps16-rrn16_p3	TGCTCAACCTACAGAAACGG	TCCGACTTGCATGTGTCAAG	50.0	50.0	57.8	58.5	1645-2979 bp	0.714286	73.8
rps16-rrn16_p4	GCTCAACCTACAGAAACGGT	TCCGACTTGCATGTGTCAAG	50.0	50.0	57.8	58.5	1644-2978 bp	0.714286	73.8
rps16-rrn16_p5	TGCTCAACCTACAGAAACGG	CACTGGAAACACCACTTCCC	50.0	55.0	57.8	58.7	1666-3000 bp	0.714286	72.2