primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpoB-clpP_p1	rpoB-clpP	1	TAAACCCAGGTATTGCCGAC	ATGTCAGCAAAAGAAGCCCA	20	20	50.0	45.0	57.6	58.0	0.0	0.0	0.406948	81046	83782	2832-2957	1.000000	88.7
rpoB-clpP_p2	rpoB-clpP	2	CAGGTATTGCCGACATTCCT	ATGTCAGCAAAAGAAGCCCA	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.332415	81046	83782	2852-2951	0.857143	82.2
rpoB-clpP_p3	rpoB-clpP	3	GTTCTTCCATCAAGCCCTGA	ATGTCAGCAAAAGAAGCCCA	20	20	50.0	45.0	57.5	58.0	0.0	0.0	0.497250	81046	83782	2889-2964	0.857143	81.6
rpoB-clpP_p4	rpoB-clpP	4	AGTTCTTCCATCAAGCCCTG	ATGTCAGCAAAAGAAGCCCA	20	20	50.0	45.0	57.5	58.0	0.0	0.0	0.504247	81046	83782	2890-2965	0.857143	81.5
rpoB-clpP_p5	rpoB-clpP	5	CAGGTATTGCCGACATTCCT	TGTCAGCAAAAGAAGCCCAA	20	20	50.0	45.0	57.7	58.2	0.0	0.0	0.557953	81046	83782	2851-2950	0.857143	80.4
rps16-rrn16_p1	rps16-rrn16	1	TGCTCAACCTACAGAAACGG	CCAGGATCGAACTCTCCATG	20	20	50.0	55.0	57.8	57.5	0.0	5.6	0.686654	109644	112452	1601-2980	0.857143	79.3
rps16-rrn16_p2	rps16-rrn16	2	GCTCAACCTACAGAAACGGT	CCAGGATCGAACTCTCCATG	20	20	50.0	55.0	57.8	57.5	0.0	5.6	0.688154	109644	112452	1600-2979	0.857143	79.3
rps16-rrn16_p3	rps16-rrn16	3	TGCTCAACCTACAGAAACGG	TCCGACTTGCATGTGTCAAG	20	20	50.0	50.0	57.8	58.5	0.0	10.4	0.651968	109644	112452	1645-2979	0.714286	73.8
rps16-rrn16_p4	rps16-rrn16	4	GCTCAACCTACAGAAACGGT	TCCGACTTGCATGTGTCAAG	20	20	50.0	50.0	57.8	58.5	0.0	10.4	0.653468	109644	112452	1644-2978	0.714286	73.8
rps16-rrn16_p5	rps16-rrn16	5	TGCTCAACCTACAGAAACGG	CACTGGAAACACCACTTCCC	20	20	50.0	55.0	57.8	58.7	0.0	0.0	0.845242	109644	112452	1666-3000	0.714286	72.2
trnI-GAU_p1	trnI-GAU	1	AACTCCAAAAACCCGTCCTC	CCTCTACGTTCTTGACAGCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.025275	114244	115261	1829-1840	1.000000	91.8
trnI-GAU_p2	trnI-GAU	2	AACTCCAAAAACCCGTCCTC	CTCTACGTTCTTGACAGCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.025275	114244	115261	1828-1839	1.000000	91.8
trnI-GAU_p3	trnI-GAU	3	GACCGGAGTGAAGTCGTAAC	CCTCTACGTTCTTGACAGCC	20	20	55.0	55.0	58.0	58.0	9.5	0.0	0.027844	114244	115261	1627-1637	1.000000	91.8
trnI-GAU_p4	trnI-GAU	4	GACCGGAGTGAAGTCGTAAC	CTCTACGTTCTTGACAGCCC	20	20	55.0	55.0	58.0	58.0	9.5	0.0	0.027844	114244	115261	1626-1636	1.000000	91.8
trnI-GAU_p5	trnI-GAU	5	TGCCATCGTTGAGTTTGGAA	CTCTACGTTCTTGACAGCCC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.028066	114244	115261	1980-1991	1.000000	91.8
rrn5-trnR-ACG_p1	rrn5-trnR-ACG	1	CCTTGTTCCTACATGACCCG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026507	119765	120012	1271-1275	1.000000	91.8
rrn5-trnR-ACG_p2	rrn5-trnR-ACG	2	TGTCCCCTCCAGTCAAGAAT	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.047124	119765	120012	1111-1115	1.000000	91.6
rrn5-trnR-ACG_p3	rrn5-trnR-ACG	3	CAGCTGAGGCATCCTAACAG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	58.0	58.0	8.4	0.0	0.051503	119765	120012	1306-1310	1.000000	91.6
rrn5-trnR-ACG_p4	rrn5-trnR-ACG	4	CCTTGTTCCTACATGACCCG	TAGCGCATCAAAGATGCAGT	20	20	55.0	45.0	58.0	58.0	0.0	25.9	0.052595	119765	120012	1048-1052	1.000000	91.6
rrn5-trnR-ACG_p5	rrn5-trnR-ACG	5	TGCCTCTCCAAGGGATGTAA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.036917	119765	120012	1149-1151	0.285714	51.3
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CTCGACGCCAGGATGTTAAA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.006913	120087	120599	924-928	1.000000	91.9
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CTCGACGCCAGGATGTTAAA	CAGTACCCTCGTTGACTTCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.026041	120087	120599	1221-1225	1.000000	91.8
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CCTTGTTCCTACATGACCCG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026507	120087	120599	1271-1275	1.000000	91.8
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CCTTGTTCCTACATGACCCG	CAGTACCCTCGTTGACTTCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.045634	120087	120599	1568-1572	1.000000	91.6
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	TGCCTCTCCAAGGGATGTAA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.036917	120087	120599	1149-1151	0.285714	51.3
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	GGCAGCGAATCGAACAAAAA	TACCCGTCTCTGGTTGTCTT	20	20	45.0	50.0	58.0	58.0	6.9	0.0	0.050258	123916	124035	538-1983	0.857143	85.9
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	TATTCGACTGGAATGGCAGC	TACCCGTCTCTGGTTGTCTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051811	123916	124035	552-1997	0.857143	85.9
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	TCGTCCGCTTGACAATACTG	TTTTTGTTCGATTCGCTGCC	20	20	50.0	45.0	58.0	58.0	4.4	0.0	0.054089	123916	124035	869-1155	0.571429	74.4
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	TCGTCCGCTTGACAATACTG	GAGCGGCCTATTGTAGATCG	20	20	50.0	55.0	58.0	58.0	4.4	0.0	0.025228	123916	124035	357-1789	0.571429	73.7
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	TCGTCCGCTTGACAATACTG	AGTCCACCTCCAATCACTGA	20	20	50.0	50.0	58.0	58.0	4.4	0.0	0.039228	123916	124035	179-1611	0.571429	71.1
ndhI-ndhG_p1	ndhI-ndhG	1	AGGCAATGGAATAAAGCATCA	AAGACCCAATCCCAGGAAGA	21	20	38.1	50.0	55.9	57.9	0.0	0.0	3.176889	131036	133582	1408-2643	0.714286	49.6
ndhI-ndhG_p2	ndhI-ndhG	2	CGTCTCATCATAAAAGGCAATGG	AAGACCCAATCCCAGGAAGA	23	20	43.5	50.0	58.1	57.9	0.0	0.0	3.207618	131036	133582	1422-2657	0.714286	49.3
ndhI-ndhG_p3	ndhI-ndhG	3	GGCAATGGAATAAAGCATCAAGA	AAGACCCAATCCCAGGAAGA	23	20	39.1	50.0	57.7	57.9	0.0	0.0	3.326490	131036	133582	1407-2642	0.714286	48.4
ndhI-ndhG_p4	ndhI-ndhG	4	AGGCAATGGAATAAAGCATCAAG	AAGACCCAATCCCAGGAAGA	23	20	39.1	50.0	57.7	57.9	0.0	0.0	3.332039	131036	133582	1408-2643	0.714286	48.4
ndhI-ndhG_p5	ndhI-ndhG	5	AGGCAATGGAATAAAGCATCAA	AAGACCCAATCCCAGGAAGA	22	20	36.4	50.0	56.6	57.9	0.0	0.0	3.425853	131036	133582	1408-2643	0.714286	47.6
rpl32-ndhF_p1	rpl32-ndhF	1	AGCGTGTCTACCGATTTCAC	GGGAATTGCGCAGATTTAGC	20	20	50.0	50.0	58.0	57.9	0.0	11.3	0.133292	138854	140254	1839-2866	0.857143	82.2
rpl32-ndhF_p2	rpl32-ndhF	2	AGCGTGTCTACCGATTTCAC	TACGTGAAGAGGGAATTGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.012413	138854	140254	1849-2876	0.714286	75.0
rpl32-ndhF_p3	rpl32-ndhF	3	CAGCGTGTCTACCGATTTCA	TACGTGAAGAGGGAATTGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013783	138854	140254	1850-2877	0.714286	75.0
rpl32-ndhF_p4	rpl32-ndhF	4	TCCTAAGAGCAGCGTGTCTA	TACGTGAAGAGGGAATTGCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.128860	138854	140254	1859-2886	0.714286	74.1
rpl32-ndhF_p5	rpl32-ndhF	5	AGCTTTTAAGGCTGCCCAAT	TACGTGAAGAGGGAATTGCG	20	20	45.0	50.0	58.1	58.0	16.6	0.0	0.061806	138854	140254	2215-2238	0.571429	65.9
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCAATCCCAGGAAGA	AGGCAATGGAATAAAGCATCA	20	21	50.0	38.1	57.9	55.9	0.0	0.0	3.176889	148563	151109	1408-2643	0.714286	49.6
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCAATCCCAGGAAGA	CGTCTCATCATAAAAGGCAATGG	20	23	50.0	43.5	57.9	58.1	0.0	0.0	3.207618	148563	151109	1422-2657	0.714286	49.3
ndhG-ndhI_p3	ndhG-ndhI	3	AAGACCCAATCCCAGGAAGA	GGCAATGGAATAAAGCATCAAGA	20	23	50.0	39.1	57.9	57.7	0.0	0.0	3.326490	148563	151109	1407-2642	0.714286	48.4
ndhG-ndhI_p4	ndhG-ndhI	4	AAGACCCAATCCCAGGAAGA	AGGCAATGGAATAAAGCATCAAG	20	23	50.0	39.1	57.9	57.7	0.0	0.0	3.332039	148563	151109	1408-2643	0.714286	48.4
ndhG-ndhI_p5	ndhG-ndhI	5	AAGACCCAATCCCAGGAAGA	AGGCAATGGAATAAAGCATCAA	20	22	50.0	36.4	57.9	56.6	0.0	0.0	3.425853	148563	151109	1408-2643	0.714286	47.6
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	TCCCGCAAAAATTCTGGACA	AAGACAACCAGAGACGGGTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.017077	157034	157153	1760-1871	0.714286	80.4
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	CGACTCGACAAGACAACGAT	TCAGTGATTGGAGGTGGACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.064944	157034	157153	1624-1731	0.571429	73.3
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	ATACAAAGGAGAGCCCCTGT	TCAGTGATTGGAGGTGGACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057439	157034	157153	1396-1412	0.428571	66.4
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	TCCCGCAAAAATTCTGGACA	CGATCTACAATAGGCCGCTC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.027734	157034	157153	1694-1801	0.428571	65.7
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	TCCCGCAAAAATTCTGGACA	TCAGTGATTGGAGGTGGACT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041734	157034	157153	1872-1979	0.428571	65.2
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CTCGACGCCAGGATGTTAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006913	161546	162058	924-928	1.000000	91.9
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CAGTACCCTCGTTGACTTCG	CTCGACGCCAGGATGTTAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.026041	161546	162058	1221-1225	1.000000	91.8
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CAGTACCCTCGTTGACTTCG	CCTTGTTCCTACATGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.045634	161546	162058	1568-1572	1.000000	91.6
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CCGTTGTTCCTCAGTAGCTC	CCTTGTTCCTACATGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026507	161546	162058	1271-1275	1.000000	91.1
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CCGTTGTTCCTCAGTAGCTC	TGCCTCTCCAAGGGATGTAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.036917	161546	162058	1149-1151	0.285714	51.3
trnR-ACG-rrn5_p1	trnR-ACG-rrn5	1	CCGTTGTTCCTCAGTAGCTC	CAGCTGAGGCATCCTAACAG	20	20	55.0	55.0	58.0	58.0	0.0	8.4	0.051503	162133	162380	1306-1310	1.000000	91.4
trnR-ACG-rrn5_p2	trnR-ACG-rrn5	2	CCGTTGTTCCTCAGTAGCTC	CCTTGTTCCTACATGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.026507	162133	162380	1271-1275	1.000000	91.1
trnR-ACG-rrn5_p3	trnR-ACG-rrn5	3	CCGTTGTTCCTCAGTAGCTC	TGTCCCCTCCAGTCAAGAAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.047124	162133	162380	1111-1115	1.000000	91.1
trnR-ACG-rrn5_p4	trnR-ACG-rrn5	4	TAGCGCATCAAAGATGCAGT	CCTTGTTCCTACATGACCCG	20	20	45.0	55.0	58.0	58.0	25.9	0.0	0.052595	162133	162380	1048-1052	1.000000	90.9
trnR-ACG-rrn5_p5	trnR-ACG-rrn5	5	CCGTTGTTCCTCAGTAGCTC	TGCCTCTCCAAGGGATGTAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.036917	162133	162380	1149-1151	0.285714	51.3
trnI-GAU_p1	trnI-GAU	1	CCTCTACGTTCTTGACAGCC	AACTCCAAAAACCCGTCCTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025275	166884	167901	1829-1840	1.000000	91.8
trnI-GAU_p2	trnI-GAU	2	CTCTACGTTCTTGACAGCCC	AACTCCAAAAACCCGTCCTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025275	166884	167901	1828-1839	1.000000	91.8
trnI-GAU_p3	trnI-GAU	3	CCTCTACGTTCTTGACAGCC	GACCGGAGTGAAGTCGTAAC	20	20	55.0	55.0	58.0	58.0	0.0	9.5	0.027844	166884	167901	1627-1637	1.000000	91.8
trnI-GAU_p4	trnI-GAU	4	CTCTACGTTCTTGACAGCCC	GACCGGAGTGAAGTCGTAAC	20	20	55.0	55.0	58.0	58.0	0.0	9.5	0.027844	166884	167901	1626-1636	1.000000	91.8
trnI-GAU_p5	trnI-GAU	5	CTCTACGTTCTTGACAGCCC	TGCCATCGTTGAGTTTGGAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.028066	166884	167901	1980-1991	1.000000	91.8
rrn16-rps16_p1	rrn16-rps16	1	CCAGGATCGAACTCTCCATG	TGCTCAACCTACAGAAACGG	20	20	55.0	50.0	57.5	57.8	5.6	0.0	0.686654	169693	172501	1601-2980	0.857143	79.6
rrn16-rps16_p2	rrn16-rps16	2	CCAGGATCGAACTCTCCATG	GCTCAACCTACAGAAACGGT	20	20	55.0	50.0	57.5	57.8	5.6	0.0	0.688154	169693	172501	1600-2979	0.857143	79.6
rrn16-rps16_p3	rrn16-rps16	3	TCCGACTTGCATGTGTCAAG	TGCTCAACCTACAGAAACGG	20	20	50.0	50.0	58.5	57.8	10.4	0.0	0.651968	169693	172501	1645-2979	0.714286	74.1
rrn16-rps16_p4	rrn16-rps16	4	TCCGACTTGCATGTGTCAAG	GCTCAACCTACAGAAACGGT	20	20	50.0	50.0	58.5	57.8	10.4	0.0	0.653468	169693	172501	1644-2978	0.714286	74.1
rrn16-rps16_p5	rrn16-rps16	5	CACTGGAAACACCACTTCCC	TGCTCAACCTACAGAAACGG	20	20	55.0	50.0	58.7	57.8	0.0	0.0	0.845242	169693	172501	1666-3000	0.714286	72.5