Hotspot

rps8-infA

IRa · 185970-186740 · 771 bp

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Pi 0.0834
MarkerSeek score 79.00
Primer available yes
Label rps8-infA

Feature Metadata

label_name
rps8-infA
feature_type
igs
region
IRa
length_bp
771
variable_sites
64
pi
0.0834
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-infA_p1 AAGGAATTGGAGGGGAGGTT TCTTTTTCTTCTTTTTCTGTCA 50.0 27.3 58.2 52.1 832-1346 bp 1.0 45.0
rps8-infA_p2 AGGAATTGGAGGGGAGGTTT TCTTTTTCTTCTTTTTCTGTCA 50.0 27.3 58.2 52.1 831-1345 bp 1.0 45.0
rps8-infA_p3 ATTGGAGGGGAGGTTTTGTG TCTTTTTCTTCTTTTTCTGTCA 50.0 27.3 57.7 52.1 827-1341 bp 1.0 45.0
rps8-infA_p4 AAGGAATTGGAGGGGAGGTT TTCTTTTTCTTCTTTTTCTGTCA 50.0 26.1 58.2 53.0 833-1347 bp 1.0 45.0
rps8-infA_p5 AGGAATTGGAGGGGAGGTTT TTCTTTTTCTTCTTTTTCTGTCA 50.0 26.1 58.2 53.0 832-1346 bp 1.0 45.0