Hotspot

ndhF-rpl32

SSC · 137554-138792 · 1239 bp

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Pi 0.0526
MarkerSeek score 77.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1239
variable_sites
61
pi
0.0526
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTCGATTAAGTGGCCGAACT CATAAAGGGAGCGTCGTTCA 50.0 50.0 58.0 58.0 2008-2044 bp 1.0 86.2
ndhF-rpl32_p2 GTCGATTAAGTGGCCGAACT AAAGGGAGCGTCGTTCATAC 50.0 50.0 58.0 58.0 2005-2041 bp 1.0 86.2
ndhF-rpl32_p3 GTCGATTAAGTGGCCGAACT GGAGCGTCGTTCATACTTGT 50.0 50.0 58.0 58.0 2001-2037 bp 1.0 86.2
ndhF-rpl32_p4 GGAAACTGTGCAGATTTCGC CATAAAGGGAGCGTCGTTCA 50.0 50.0 58.0 58.0 2472-2508 bp 1.0 85.0
ndhF-rpl32_p5 GGAAACTGTGCAGATTTCGC AAAGGGAGCGTCGTTCATAC 50.0 50.0 58.0 58.0 2469-2505 bp 1.0 84.9