Hotspot

infA-rps8

IRb · 87670-88440 · 771 bp

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Pi 0.0834
MarkerSeek score 79.00
Primer available yes
Label infA-rps8

Feature Metadata

label_name
infA-rps8
feature_type
igs
region
IRb
length_bp
771
variable_sites
64
pi
0.0834
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA-rps8_p1 TCTTTTTCTTCTTTTTCTGTCA AAGGAATTGGAGGGGAGGTT 27.3 50.0 52.1 58.2 832-1346 bp 1.0 45.0
infA-rps8_p2 TCTTTTTCTTCTTTTTCTGTCA AGGAATTGGAGGGGAGGTTT 27.3 50.0 52.1 58.2 831-1345 bp 1.0 45.0
infA-rps8_p3 TCTTTTTCTTCTTTTTCTGTCA ATTGGAGGGGAGGTTTTGTG 27.3 50.0 52.1 57.7 827-1341 bp 1.0 45.0
infA-rps8_p4 TTCTTTTTCTTCTTTTTCTGTCA AAGGAATTGGAGGGGAGGTT 26.1 50.0 53.0 58.2 833-1347 bp 1.0 45.0
infA-rps8_p5 TTCTTTTTCTTCTTTTTCTGTCA AGGAATTGGAGGGGAGGTTT 26.1 50.0 53.0 58.2 832-1346 bp 1.0 45.0