Hotspot

psbC-trnS-UGA

LSC · 38795-39216 · 422 bp

Back to Uvaria

Pi 0.0651
MarkerSeek score 87.10
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
422
variable_sites
27
pi
0.0651
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CATTTGTGGCATGCAGGAAG GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.0 58.1 598-601 bp 1.0 84.0
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GGTTGATAGCTCCGGTCTTG 55.0 55.0 58.2 58.1 526-529 bp 1.0 83.4
psbC-trnS-UGA_p3 CATTTGTGGCATGCAGGAAG GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.0 58.2 544-547 bp 1.0 83.2
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.2 58.2 472-475 bp 1.0 82.7
psbC-trnS-UGA_p5 TCATTTGTGGCATGCAGGAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 58.4 58.1 599-602 bp 1.0 81.1