Hotspot

petN-psbM

LSC · 31349-32187 · 839 bp

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Pi 0.0179
MarkerSeek score 67.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
839
variable_sites
15
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ATGGGGAAGAAGTGGACTCT TGCTACTGCGTTGTTCATTCT 50.0 42.9 57.6 58.2 940 bp 1.0 68.6
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCGTTGTTCATTC 50.0 45.0 57.6 56.7 940 bp 1.0 68.5
petN-psbM_p3 GGCTGCTTTGATGGTAGTCT TGCTACTGCGTTGTTCATTCT 50.0 42.9 57.6 58.2 985 bp 1.0 68.1
petN-psbM_p4 GGCTGCTTTGATGGTAGTCT TGCTACTGCGTTGTTCATTC 50.0 45.0 57.6 56.7 985 bp 1.0 67.9
petN-psbM_p5 GGGCTGCTTTGATGGTAGTC TGCTACTGCGTTGTTCATTCT 55.0 42.9 58.6 58.2 986 bp 1.0 66.4