Hotspot

rpoC1-rpoB

LSC · 25463-25488 · 26 bp

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Pi 0.0769
MarkerSeek score 75.50
Primer available yes
Label rpoC1-rpoB

Feature Metadata

label_name
rpoC1-rpoB
feature_type
igs
region
LSC
length_bp
26
variable_sites
2
pi
0.0769
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-rpoB_p1 TTTGGCCCAGGCACTAATTT GATCCTTGGCTCTGGAACTG 45.0 55.0 58.0 58.0 175 bp 1.0 80.4
rpoC1-rpoB_p2 TTTTGGCCCAGGCACTAATT GATCCTTGGCTCTGGAACTG 45.0 55.0 58.0 58.0 176 bp 1.0 80.4
rpoC1-rpoB_p3 TTTTTGGCCCAGGCACTAAT GATCCTTGGCTCTGGAACTG 45.0 55.0 58.0 58.0 177 bp 1.0 80.4
rpoC1-rpoB_p4 TTTGGCCCAGGCACTAATTT TTGCTCGTTCGAGAACTACG 45.0 50.0 58.0 58.0 194 bp 1.0 80.3
rpoC1-rpoB_p5 TTTTGGCCCAGGCACTAATT TTGCTCGTTCGAGAACTACG 45.0 50.0 58.0 58.0 195 bp 1.0 80.3