Hotspot

atpH-atpI

LSC · 15354-16444 · 1091 bp

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Pi 0.0165
MarkerSeek score 68.20
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1091
variable_sites
18
pi
0.0165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT AGGCGAATCCATGGAAAGTC 45.0 50.0 58.0 58.0 1151 bp 1.0 80.6
atpH-atpI_p2 TACCTTGACCAACTCCAGGT AGGCGAATCCATGGAAAGTC 50.0 50.0 57.9 58.0 1207 bp 1.0 79.9
atpH-atpI_p3 AATAACGGAAGCGGCAGAAA AGGCGAATCCATGGAAAGTC 45.0 50.0 58.2 58.0 1152 bp 1.0 79.4
atpH-atpI_p4 GCAGTACCTTGACCAACTCC AGGCGAATCCATGGAAAGTC 55.0 50.0 58.2 58.0 1211 bp 1.0 79.3
atpH-atpI_p5 GGCAGTACCTTGACCAACTC AGGCGAATCCATGGAAAGTC 55.0 50.0 58.2 58.0 1212 bp 1.0 79.3