Hotspot

ccsA

SSC · 102785-103753 · 969 bp

Back to Utricularia

Pi 0.101

MarkerSeek score 83.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 401
pi: 0.101
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCTACCCCAAACCTCCAAAA	TGATACCCTGGGATGGTTGT	50.0	50.0	58.0	58.0	1975 bp	0.04	41.4
ccsA_p2	CTTTCAACGGGGAGTTCCAA	TGATACCCTGGGATGGTTGT	50.0	50.0	58.0	58.0	2080 bp	0.04	41.2
ccsA_p3	TCAACGGGGAGTTCCAAAAG	TGATACCCTGGGATGGTTGT	50.0	50.0	58.0	58.0	2077 bp	0.04	41.2
ccsA_p4	GCTACCCCAAACCTCCAAAA	AGATGTGAATCCGCATGTCC	50.0	50.0	58.0	58.0	1901 bp	0.04	41.2
ccsA_p5	TCAACGGGGAGTTCCAAAAG	AGATGTGAATCCGCATGTCC	50.0	50.0	58.0	58.0	2003 bp	0.04	41.1