Hotspot

rpl32-trnL-UAG

SSC · 102120-102623 · 504 bp

Back to Utricularia

Pi 0.266

MarkerSeek score 74.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 504
variable_sites: 379
pi: 0.266
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTTTCAACGGGGAGTTCCAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	609 bp	0.04	40.4
rpl32-trnL-UAG_p2	TCAACGGGGAGTTCCAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	606 bp	0.04	40.4
rpl32-trnL-UAG_p3	CTTTCAACGGGGAGTTCCAA	CGATCCACATAACAGTCCCG	50.0	55.0	58.0	58.1	1193 bp	0.04	40.2
rpl32-trnL-UAG_p4	TCAACGGGGAGTTCCAAAAG	CGATCCACATAACAGTCCCG	50.0	55.0	58.0	58.1	1190 bp	0.04	40.2
rpl32-trnL-UAG_p5	TCAACGGGGAGTTCCAAAAG	AAGCCCTGGGTAGAAATTGC	50.0	50.0	58.0	58.1	1063 bp	0.04	40.2