Hotspot

rpl20-rps12

LSC · 63406-64207 · 802 bp

Back to Utricularia

Pi 0.0949

MarkerSeek score 83.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 314
pi: 0.0949
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGCACGTTTAATCCTGGTCA	CTCAACGTCGAGGAACATGT	45.0	50.0	57.4	57.9	786-937 bp	0.84	80.7
rpl20-rps12_p2	ATGCACGTTTAATCCTGGTCA	CTCAACGTCGAGGAACATGT	42.9	50.0	57.9	57.9	787-938 bp	0.84	76.5
rpl20-rps12_p3	TGCACGTTTAATCCTGGTCAT	CTCAACGTCGAGGAACATGT	42.9	50.0	57.9	57.9	786-937 bp	0.84	76.5
rpl20-rps12_p4	TTGTTCTACGTCTTCGAGCT	CTCAACGTCGAGGAACATGT	45.0	50.0	56.6	57.9	807-962 bp	0.88	75.7
rpl20-rps12_p5	TGCACGTTTAATCCTGGTCA	CAACGTCGAGGAACATGTACT	45.0	47.6	57.4	58.1	784-935 bp	0.84	72.7