Hotspot

rbcL-accD

LSC · 52285-52945 · 661 bp

Back to Utricularia

Pi 0.13

MarkerSeek score 82.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 359
pi: 0.13
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGAATGCTACTGCGGGTAC	CCCTACCCTTTCGATTTGCA	50.0	50.0	58.0	57.9	1537-1648 bp	0.48	70.1
rbcL-accD_p2	AATGCTACTGCGGGTACATG	CCCTACCCTTTCGATTTGCA	50.0	50.0	58.0	57.9	1534-1645 bp	0.48	69.8
rbcL-accD_p3	CGTGGCTTCAGGAGGTATTC	CCCTACCCTTTCGATTTGCA	55.0	50.0	58.1	57.9	1127-1183 bp	0.28	61.7
rbcL-accD_p4	GGCTTCAGGAGGTATTCACG	CCCTACCCTTTCGATTTGCA	55.0	50.0	58.1	57.9	1124-1180 bp	0.28	61.7
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	CCCGCCGTCATTACTAGTTC	50.0	55.0	58.0	58.2	1992-2018 bp	0.16	56.4