Hotspot

atpB-rbcL

LSC · 50092-50850 · 759 bp

Back to Utricularia

Pi 0.102
MarkerSeek score 84.40
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
759
variable_sites
337
pi
0.102
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CTACATCTAGTACCGGGCCA AGAAACGATCTCTCCAACGC 55.0 50.0 58.0 58.0 1493-1549 bp 0.4 67.9
atpB-rbcL_p2 CTACATCTAGTACCGGGCCA GTAAGTCCATCGGTCCACAC 55.0 55.0 58.0 58.0 1062-1118 bp 0.4 67.9
atpB-rbcL_p3 CTACATCTAGTACCGGGCCA GTACCCGCAGTAGCATTCAA 55.0 50.0 58.0 58.0 1575-1631 bp 0.4 67.8
atpB-rbcL_p4 CTACATCTAGTACCGGGCCA AGATCCTCCAGACGTAGAGC 55.0 55.0 58.0 58.0 1251-1307 bp 0.4 67.7
atpB-rbcL_p5 CTACATCTAGTACCGGGCCA GAGGACGACCGTACTTGTTC 55.0 55.0 58.0 58.0 1364 bp 0.04 41.3