Hotspot

trnT-GGU-psbD

LSC · 30412-31747 · 1336 bp

Back to Utricularia

Pi 0.129

MarkerSeek score 86.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1336
variable_sites: 706
pi: 0.129
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTAGAGTCA	55.0	45.0	58.1	55.5	1206-1578 bp	0.92	68.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTAGAGTCA	50.0	45.0	58.1	55.5	1209-1581 bp	0.92	67.8
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTAGAGTCA	50.0	45.0	58.3	55.5	1183-1555 bp	0.92	66.3
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTAGAGTCAT	55.0	45.0	58.1	55.2	1205-1577 bp	0.92	66.1
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TTACCAAGGGCTAGAGTCAT	50.0	45.0	58.1	55.2	1208-1580 bp	0.92	65.9