Hotspot

trnS-GCU-trnG-UCC

LSC · 7951-8596 · 646 bp

Back to Utricularia

Pi 0.216

MarkerSeek score 81.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 450
pi: 0.216
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	622-1175 bp	1.0	63.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	624-1177 bp	1.0	62.6
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	623-1176 bp	0.96	62.1
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	626-1179 bp	0.96	61.0
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	625-1178 bp	0.96	61.0