primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	TTGCAATTCCAAGACTCGCT	CGTGATAACGGAACAGGGTT	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.192730	3441	4132	1656-1656	0.040000	40.1
matK-trnK-UUU_p2	matK-trnK-UUU	2	TTGCAATTCCAAGACTCGCT	TCGGGTTGCTAACTCAATGG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.211730	3441	4132	1282-1282	0.040000	39.9
matK-trnK-UUU_p3	matK-trnK-UUU	3	TTGCAATTCCAAGACTCGCT	GGAACAGGGTTGCGTCTATT	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.213205	3441	4132	1647-1647	0.040000	39.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	TTGCAATTCCAAGACTCGCT	CGGGTTGCTAACTCAATGGT	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.282564	3441	4132	1281-1281	0.040000	39.3
matK-trnK-UUU_p5	matK-trnK-UUU	5	TTGCAATTCCAAGACTCGCT	TGATAACGGAACAGGGTTGC	20	20	45.0	50.0	58.1	58.2	0.0	0.0	0.287706	3441	4132	1654-1654	0.040000	39.3
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AACGGCAGGCAATAGTTAGG	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.046629	4170	5052	1876-2065	0.640000	77.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AACGGCAGGCAATAGTTAGG	TCGATCTCGACGAGAAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	29.7	0.058335	4170	5052	1481-1571	0.440000	69.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TTACACATAGAACGGCAGGC	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.026784	4170	5052	2019-2076	0.160000	58.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TTACACATAGAACGGCAGGC	TCGATCTCGACGAGAAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	29.7	0.038490	4170	5052	1535-1561	0.120000	56.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TTACACATAGAACGGCAGGC	TAGTCAACGTGGATCATGCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.097298	4170	5052	2081-2081	0.040000	40.8
rps16_p1	rps16	1	AACCCTGTTCCGTTATCACG	CTACTGTGTACCGCTGAACC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.096780	5053	6147	1705-1705	0.040000	40.8
rps16_p2	rps16	2	AATAGACGCAACCCTGTTCC	CTACTGTGTACCGCTGAACC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.117255	5053	6147	1714-1714	0.040000	40.7
rps16_p3	rps16	3	AACCCTGTTCCGTTATCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.165051	5053	6147	2160-2160	0.040000	40.3
rps16_p4	rps16	4	AACCCTGTTCCGTTATCACG	GTACCGCTGAACCAATGACT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.183712	5053	6147	1698-1698	0.040000	40.1
rps16_p5	rps16	5	AATAGACGCAACCCTGTTCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	0.0	16.9	0.185526	5053	6147	2169-2169	0.040000	40.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TGGCAGCAACATACCCTTTT	CTTACAGCTGCTTGCCAAAC	20	20	45.0	50.0	58.0	57.9	0.0	15.3	0.064337	6148	6661	1382-1891	0.760000	81.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TGGCAGCAACATACCCTTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.0	58.1	0.0	16.9	0.084343	6148	6661	829-1294	0.720000	80.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AGATCCTTGTCCCCGAGAAA	CTTACAGCTGCTTGCCAAAC	20	20	50.0	50.0	58.0	57.9	0.0	15.3	0.103473	6148	6661	1200-1688	0.640000	76.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AGCATCCTCATTTCGCGATT	CTTACAGCTGCTTGCCAAAC	20	20	45.0	50.0	58.0	57.9	10.9	15.3	0.097053	6148	6661	1243-1243	0.040000	40.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGCATCCTCATTTCGCGATT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.0	58.1	10.9	16.9	0.117059	6148	6661	654-654	0.040000	40.7
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	7951	8596	622-1175	1.000000	63.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	7951	8596	624-1177	1.000000	62.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	7951	8596	623-1176	0.960000	62.1
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	7951	8596	626-1179	0.960000	61.0
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	7951	8596	625-1178	0.960000	61.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TTTTCTACTAACGCGAGCCC	TTGCAAGCCTTGGTCTTGAT	20	20	50.0	45.0	58.0	58.0	15.9	20.0	0.000781	25935	27085	1976-1976	0.040000	41.6
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATACTGGATCCCCACCTACG	TTGCAAGCCTTGGTCTTGAT	20	20	55.0	45.0	58.0	58.0	9.9	20.0	0.011474	25935	27085	2166-2166	0.040000	41.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCACCTACGCAAGCAAATTG	TTGCAAGCCTTGGTCTTGAT	20	20	50.0	45.0	58.0	58.0	0.0	20.0	0.019098	25935	27085	2155-2155	0.040000	41.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CGAGCCCATATCCTTGCTTT	TTGCAAGCCTTGGTCTTGAT	20	20	50.0	45.0	58.0	58.0	0.0	20.0	0.027480	25935	27085	1963-1963	0.040000	41.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCTACTAACGCGAGCCCATA	TTGCAAGCCTTGGTCTTGAT	20	20	50.0	45.0	58.0	58.0	15.9	20.0	0.028871	25935	27085	1973-1973	0.040000	41.4
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTAGAGTCA	20	20	55.0	45.0	58.1	55.5	7.6	0.0	2.599644	30412	31747	1206-1578	0.920000	68.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTAGAGTCA	20	20	50.0	45.0	58.1	55.5	0.0	0.0	2.621798	30412	31747	1209-1581	0.920000	67.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTAGAGTCA	20	20	50.0	45.0	58.3	55.5	0.0	0.0	2.818382	30412	31747	1183-1555	0.920000	66.3
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTAGAGTCAT	20	20	55.0	45.0	58.1	55.2	7.6	0.0	2.840364	30412	31747	1205-1577	0.920000	66.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	TTACCAAGGGCTAGAGTCAT	20	20	50.0	45.0	58.1	55.2	0.0	0.0	2.862519	30412	31747	1208-1580	0.920000	65.9
atpB-rbcL_p1	atpB-rbcL	1	CTACATCTAGTACCGGGCCA	AGAAACGATCTCTCCAACGC	20	20	55.0	50.0	58.0	58.0	6.3	0.0	0.011210	50092	50850	1493-1549	0.400000	67.9
atpB-rbcL_p2	atpB-rbcL	2	CTACATCTAGTACCGGGCCA	GTAAGTCCATCGGTCCACAC	20	20	55.0	55.0	58.0	58.0	6.3	0.0	0.014850	50092	50850	1062-1118	0.400000	67.9
atpB-rbcL_p3	atpB-rbcL	3	CTACATCTAGTACCGGGCCA	GTACCCGCAGTAGCATTCAA	20	20	55.0	50.0	58.0	58.0	6.3	0.0	0.027421	50092	50850	1575-1631	0.400000	67.8
atpB-rbcL_p4	atpB-rbcL	4	CTACATCTAGTACCGGGCCA	AGATCCTCCAGACGTAGAGC	20	20	55.0	55.0	58.0	58.0	6.3	0.0	0.033219	50092	50850	1251-1307	0.400000	67.7
atpB-rbcL_p5	atpB-rbcL	5	CTACATCTAGTACCGGGCCA	GAGGACGACCGTACTTGTTC	20	20	55.0	55.0	58.0	58.0	6.3	8.8	0.034392	50092	50850	1364-1364	0.040000	41.3
rbcL-accD_p1	rbcL-accD	1	TTGAATGCTACTGCGGGTAC	CCCTACCCTTTCGATTTGCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.141325	52285	52945	1537-1648	0.480000	70.1
rbcL-accD_p2	rbcL-accD	2	AATGCTACTGCGGGTACATG	CCCTACCCTTTCGATTTGCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.169224	52285	52945	1534-1645	0.480000	69.8
rbcL-accD_p3	rbcL-accD	3	CGTGGCTTCAGGAGGTATTC	CCCTACCCTTTCGATTTGCA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.181458	52285	52945	1127-1183	0.280000	61.7
rbcL-accD_p4	rbcL-accD	4	GGCTTCAGGAGGTATTCACG	CCCTACCCTTTCGATTTGCA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.181458	52285	52945	1124-1180	0.280000	61.7
rbcL-accD_p5	rbcL-accD	5	TTGAATGCTACTGCGGGTAC	CCCGCCGTCATTACTAGTTC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.169445	52285	52945	1992-2018	0.160000	56.4
accD_p1	accD	1	TGTCTTTTTCGTTCCGTGGT	AAAGAAGCCATCGCAATTGC	20	20	45.0	45.0	58.0	58.0	0.0	20.8	0.027433	52946	54457	2348-2514	0.360000	64.4
accD_p2	accD	2	TGTCTTTTTCGTTCCGTGGT	ACATCTGCATTCATCGACCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.067962	52946	54457	2603-2603	0.040000	41.1
accD_p3	accD	3	TGTCTTTTTCGTTCCGTGGT	GCATTCATCGACCCTGATGT	20	20	45.0	50.0	58.0	58.0	0.0	3.9	0.067962	52946	54457	2597-2597	0.040000	41.1
accD_p4	accD	4	TGTCTTTTTCGTTCCGTGGT	TGCATTCATCGACCCTGATG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.069335	52946	54457	2598-2598	0.040000	41.0
accD_p5	accD	5	TCCACAATTAAACCCACGGG	AAAGAAGCCATCGCAATTGC	20	20	50.0	45.0	58.1	58.0	0.0	20.8	0.088012	52946	54457	2460-2460	0.040000	40.9
rpl20-rps12_p1	rpl20-rps12	1	TGCACGTTTAATCCTGGTCA	CTCAACGTCGAGGAACATGT	20	20	45.0	50.0	57.4	57.9	0.0	12.9	0.618592	63406	64207	786-937	0.840000	80.7
rpl20-rps12_p2	rpl20-rps12	2	ATGCACGTTTAATCCTGGTCA	CTCAACGTCGAGGAACATGT	21	20	42.9	50.0	57.9	57.9	0.0	12.9	1.132284	63406	64207	787-938	0.840000	76.5
rpl20-rps12_p3	rpl20-rps12	3	TGCACGTTTAATCCTGGTCAT	CTCAACGTCGAGGAACATGT	21	20	42.9	50.0	57.9	57.9	0.0	12.9	1.132284	63406	64207	786-937	0.840000	76.5
rpl20-rps12_p4	rpl20-rps12	4	TTGTTCTACGTCTTCGAGCT	CTCAACGTCGAGGAACATGT	20	20	45.0	50.0	56.6	57.9	0.0	12.9	1.432972	63406	64207	807-962	0.880000	75.7
rpl20-rps12_p5	rpl20-rps12	5	TGCACGTTTAATCCTGGTCA	CAACGTCGAGGAACATGTACT	20	21	45.0	47.6	57.4	58.1	0.0	12.9	1.609890	63406	64207	784-935	0.840000	72.7
petB_p1	petB	1	ACCACCTCGAGTTCCAACTA	CTTATGCACAGCACAACACG	20	20	50.0	50.0	58.0	58.0	12.3	0.0	0.050933	69267	70656	2441-2441	0.040000	41.2
petB_p2	petB	2	GGTAAAGTAGCTCCCGGTTG	CTTATGCACAGCACAACACG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.065511	69267	70656	2012-2012	0.040000	41.1
petB_p3	petB	3	GTAAAGTAGCTCCCGGTTGG	CTTATGCACAGCACAACACG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.065511	69267	70656	2011-2011	0.040000	41.1
petB_p4	petB	4	AAGGTTGCCCAAAAGCAGTA	CTTATGCACAGCACAACACG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.126217	69267	70656	2303-2303	0.040000	40.6
petB_p5	petB	5	ACCACCTCGAGTTCCAACTA	AGATCTTACGGAGCCCACTT	20	20	50.0	50.0	58.0	58.1	12.3	0.0	0.138007	69267	70656	2458-2458	0.040000	40.5
rpoA_p1	rpoA	1	TTGGTTGGGTATTGGTGCAA	GAACTGGTTCGCGTAGGAAT	20	20	45.0	50.0	57.9	58.0	0.0	5.8	0.094075	72272	73339	1673-1713	0.760000	80.0
rpoA_p2	rpoA	2	TTTGGTTGGGTATTGGTGCA	GAACTGGTTCGCGTAGGAAT	20	20	45.0	50.0	57.9	58.0	0.0	5.8	0.094075	72272	73339	1674-1714	0.760000	80.0
rpoA_p3	rpoA	3	TTTCATGTCCGGTTCCTTGG	GAACTGGTTCGCGTAGGAAT	20	20	50.0	50.0	58.1	58.0	0.0	5.8	0.101684	72272	73339	2141-2181	0.680000	76.2
rpoA_p4	rpoA	4	TTCAATGATAGGCGAACCGG	GAACTGGTTCGCGTAGGAAT	20	20	50.0	50.0	58.1	58.0	0.0	5.8	0.057866	72272	73339	1953-1953	0.040000	41.1
rpoA_p5	rpoA	5	TTCAATGATAGGCGAACCGG	TTGTCCGAGATGTAACCCCT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.104911	72272	73339	1620-1620	0.040000	40.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CTTTCAACGGGGAGTTCCAA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.148618	102120	102623	609-609	0.040000	40.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCAACGGGGAGTTCCAAAAG	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.148618	102120	102623	606-606	0.040000	40.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CTTTCAACGGGGAGTTCCAA	CGATCCACATAACAGTCCCG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.171399	102120	102623	1193-1193	0.040000	40.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCAACGGGGAGTTCCAAAAG	CGATCCACATAACAGTCCCG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.171399	102120	102623	1190-1190	0.040000	40.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCAACGGGGAGTTCCAAAAG	AAGCCCTGGGTAGAAATTGC	20	20	50.0	50.0	58.0	58.1	0.0	15.6	0.171708	102120	102623	1063-1063	0.040000	40.2
ccsA_p1	ccsA	1	GCTACCCCAAACCTCCAAAA	TGATACCCTGGGATGGTTGT	20	20	50.0	50.0	58.0	58.0	0.0	22.2	0.024128	102785	103753	1975-1975	0.040000	41.4
ccsA_p2	ccsA	2	CTTTCAACGGGGAGTTCCAA	TGATACCCTGGGATGGTTGT	20	20	50.0	50.0	58.0	58.0	0.0	22.2	0.044531	102785	103753	2080-2080	0.040000	41.2
ccsA_p3	ccsA	3	TCAACGGGGAGTTCCAAAAG	TGATACCCTGGGATGGTTGT	20	20	50.0	50.0	58.0	58.0	0.0	22.2	0.044531	102785	103753	2077-2077	0.040000	41.2
ccsA_p4	ccsA	4	GCTACCCCAAACCTCCAAAA	AGATGTGAATCCGCATGTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044895	102785	103753	1901-1901	0.040000	41.2
ccsA_p5	ccsA	5	TCAACGGGGAGTTCCAAAAG	AGATGTGAATCCGCATGTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.065299	102785	103753	2003-2003	0.040000	41.1
rps15-ycf1_p1	rps15-ycf1	1	CGTTTGCAGCCAAAACTTCA	GGGAATGGTGCTTTGACTCA	20	20	45.0	50.0	58.1	58.1	6.7	0.0	0.170382	105484	105789	1530-1530	0.040000	40.2
rps15-ycf1_p2	rps15-ycf1	2	GTCAATCCATGCCTCTGTGT	GGGAATGGTGCTTTGACTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.210102	105484	105789	1565-1565	0.040000	39.9
rps15-ycf1_p3	rps15-ycf1	3	CGTTTGCAGCCAAAACTTCA	CTTTTTCAATTGCGCGGGAA	20	20	45.0	45.0	58.1	58.2	6.7	8.8	0.302981	105484	105789	1545-1545	0.040000	39.2
rps15-ycf1_p4	rps15-ycf1	4	GTCAATCCATGCCTCTGTGT	CTTTTTCAATTGCGCGGGAA	20	20	50.0	45.0	57.9	58.2	0.0	8.8	0.342700	105484	105789	1580-1580	0.040000	38.9
rps15-ycf1_p5	rps15-ycf1	5	TTTCTTCAATCCGTGGCAGT	GGGAATGGTGCTTTGACTCA	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.360652	105484	105789	1392-1392	0.040000	38.7
ycf1_p1	ycf1	1	TCGATTCCAGCGTTTCGAAT	TTAGGCAAAATACCCTCGCC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.051584	109886	110005	330-330	0.040000	41.2
ycf1_p2	ycf1	2	CACCACTTTCGGATATGGCA	TTAGGCAAAATACCCTCGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.083959	109886	110005	418-418	0.040000	40.9
ycf1_p3	ycf1	3	TTTCAGTTCTGGGAGCCCTA	TTAGGCAAAATACCCTCGCC	20	20	50.0	50.0	58.0	58.0	7.2	0.0	0.085865	109886	110005	382-382	0.040000	40.9
ycf1_p4	ycf1	4	AAAGGTCGATTCCAGCGTTT	TTAGGCAAAATACCCTCGCC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.154792	109886	110005	335-335	0.040000	40.4
ycf1_p5	ycf1	5	CGATGTATCGCAAAGGTCGA	TTAGGCAAAATACCCTCGCC	20	20	50.0	50.0	58.2	58.0	0.9	0.0	0.211881	109886	110005	346-346	0.040000	39.9