Hotspot

ycf1

SSC · 117762-123248 · 5487 bp

Back to Urtica

Pi 0.0359
MarkerSeek score 67.40
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5487
variable_sites
755
pi
0.0359
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CGTCGAAAATCCGGTTGTTG CTTCCCGTGGACAAATGCTA 50.0 50.0 58.1 57.9 613-622 bp 0.6 71.9
ycf1_p2 CGTCGAAAATCCGGTTGTTG TTACGCACCCACCTAGTTTG 50.0 50.0 58.1 57.8 910-919 bp 0.5 66.7
ycf1_p3 CGTCGAAAATCCGGTTGTTG TGTTTTACGCACCCACCTAG 50.0 50.0 58.1 57.8 914-923 bp 0.5 66.6
ycf1_p4 CGTCGAAAATCCGGTTGTTG CACCCACCTAGTTTGTCCAC 50.0 55.0 58.1 58.1 914 bp 0.3 52.5
ycf1_p5 ATCCTCGGACCATTCAGGAA CACCCACCTAGTTTGTCCAC 50.0 55.0 58.1 58.1 1362 bp 0.3 52.0