Hotspot

rps19

LSC · 79662-79880 · 219 bp

Pi 0.1

MarkerSeek score 64.50

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 219
variable_sites: 77
pi: 0.1
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	ATGCTCGAAACGGCATAAGT	ACTATGGGGGTGGTGAGAAA	45.0	50.0	58.0	57.9	1112-1837 bp	1.0	91.4
rps19_p2	GCTTTGTGAGCGGACATAGA	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	419-845 bp	0.85	85.2
rps19_p3	GCTTTGTGAGCGGACATAGA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	221-647 bp	0.85	85.2
rps19_p4	GCTTTGTGAGCGGACATAGA	ACTATGGGGGTGGTGAGAAA	50.0	50.0	58.0	57.9	1032-1757 bp	0.85	83.3
rps19_p5	TTTTCCTATAGCGCGCACTT	ACTATGGGGGTGGTGAGAAA	45.0	50.0	58.0	57.9	1006-1731 bp	0.7	78.9