Hotspot

rpl32-trnL-UAG

SSC · 107574-108035 · 462 bp

Back to Urtica

Pi 0.0424
MarkerSeek score 74.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
462
variable_sites
83
pi
0.0424
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTCAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 567-593 bp 1.0 89.3
rpl32-trnL-UAG_p2 GGGCAGCATTCAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 568-594 bp 1.0 89.3
rpl32-trnL-UAG_p3 GGGCAGCATTCAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 577-603 bp 1.0 88.3
rpl32-trnL-UAG_p4 GGGCAGCATTCAAAGCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.8 57.8 576-602 bp 1.0 87.8
rpl32-trnL-UAG_p5 GGGCAGCATTCAAAGCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 575-601 bp 1.0 87.8