Hotspot

ndhF-rpl32

SSC · 106650-107414 · 765 bp

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Pi 0.0599
MarkerSeek score 77.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
765
variable_sites
163
pi
0.0599
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTCCAACGCGATTAGTCACA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1833-1983 bp 0.95 89.9
ndhF-rpl32_p2 CTCCAACGCGATTAGTCACA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1834-1984 bp 0.95 89.9
ndhF-rpl32_p3 CTCCAACGCGATTAGTCACA TTAGAAGATGACATGCCGCC 50.0 50.0 58.0 58.0 1897-2047 bp 0.95 89.6
ndhF-rpl32_p4 CTCCAACGCGATTAGTCACA TAGCGGCTTGTTCGAAATCA 50.0 45.0 58.0 57.9 1811-1961 bp 0.95 89.2
ndhF-rpl32_p5 CTCCAACGCGATTAGTCACA TCGAACCGAGATGCTTTAGC 50.0 50.0 58.0 58.1 1990-2019 bp 0.75 79.2