Hotspot

psaA-ycf3

LSC · 39331-40007 · 677 bp

Back to Urtica

Pi 0.0271
MarkerSeek score 73.00
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
677
variable_sites
69
pi
0.0271
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CGGGGTCTCTCTCTACCAAA AAGATCACAAGGCGTTTCGA 55.0 45.0 58.2 57.8 743-771 bp 1.0 88.3
psaA-ycf3_p2 CGGGGTCTCTCTCTACCAAA AGATCACAAGGCGTTTCGAA 55.0 45.0 58.2 57.8 742-770 bp 1.0 88.3
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT AAGATCACAAGGCGTTTCGA 50.0 45.0 57.9 57.8 768-796 bp 1.0 88.2
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT AGATCACAAGGCGTTTCGAA 50.0 45.0 57.9 57.8 767-795 bp 1.0 88.2
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT TAGCGCTTACTCCGGGTAAT 50.0 50.0 57.9 58.3 812-840 bp 1.0 86.5