Hotspot

psbZ-trnG-GCC

LSC · 33597-33974 · 378 bp

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Pi 0.0492
MarkerSeek score 81.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
378
variable_sites
65
pi
0.0492
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CCGGTACATCATTATGGATTGGA AATCGAACCCGCATCTTCTC 43.5 50.0 58.1 58.1 499-523 bp 1.0 66.8
psbZ-trnG-GCC_p2 CCGGTACATCATTATGGATTGGA GAATCGAACCCGCATCTTCT 43.5 50.0 58.1 58.1 500-524 bp 1.0 66.8
psbZ-trnG-GCC_p3 TCCGGTACATCATTATGGATTGG AATCGAACCCGCATCTTCTC 43.5 50.0 58.1 58.1 500-524 bp 1.0 66.8
psbZ-trnG-GCC_p4 TCCGGTACATCATTATGGATTGG GAATCGAACCCGCATCTTCT 43.5 50.0 58.1 58.1 501-525 bp 1.0 66.8
psbZ-trnG-GCC_p5 CCGGTACATCATTATGGATTGG AATCGAACCCGCATCTTCTC 45.5 50.0 56.7 58.1 499-523 bp 1.0 65.2