Hotspot

trnD-GUC-trnY-GUA

LSC · 28018-28401 · 384 bp

Back to Urtica

Pi 0.0428
MarkerSeek score 75.40
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
384
variable_sites
53
pi
0.0428
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 408-458 bp 1.0 86.3
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 407-457 bp 1.0 86.3
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 429-479 bp 1.0 85.4
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 428-478 bp 1.0 85.2
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.5 57.5 424-474 bp 1.0 84.0