Hotspot

rpoB-trnC-GCA

LSC · 25033-26085 · 1053 bp

Back to Urtica

Pi 0.0424
MarkerSeek score 78.10
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1053
variable_sites
164
pi
0.0424
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TTCATTTCCACTCCCAAGCA CCGGATTTGAACTGGGGAAA 45.0 50.0 57.3 58.1 1075-1165 bp 1.0 86.0
rpoB-trnC-GCA_p2 TCATTTCCACTCCCAAGCAT CCGGATTTGAACTGGGGAAA 45.0 50.0 57.1 58.1 1074-1164 bp 1.0 84.1
rpoB-trnC-GCA_p3 TTCATTTCCACTCCCAAGCA AAAAGGATTTGCAGTCCCCC 45.0 50.0 57.3 58.4 1057-1147 bp 1.0 83.8
rpoB-trnC-GCA_p4 TTCATTTCCACTCCCAAGCAT CCGGATTTGAACTGGGGAAA 42.9 50.0 57.8 58.1 1075-1165 bp 1.0 82.0
rpoB-trnC-GCA_p5 TCATTTCCACTCCCAAGCAT AAAAGGATTTGCAGTCCCCC 45.0 50.0 57.1 58.4 1056-1146 bp 1.0 81.9