Hotspot

trnK-UUU-trnQ-UUG

LSC · 4137-5585 · 1449 bp

Back to Urtica

Pi 0.0554
MarkerSeek score 82.20
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1449
variable_sites
287
pi
0.0554
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1239-1528 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p2 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1238-1527 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p3 CCGCACTTAAAAGCCGAGTA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.3 58.1 1248-1537 bp 1.0 89.1
trnK-UUU-trnQ-UUG_p4 TACCGTTGAGTTAGCAACCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1225-1514 bp 1.0 87.7
trnK-UUU-trnQ-UUG_p5 GTGTAAAAGATGCTAGCCGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 1264-1553 bp 1.0 85.5