primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4137	5585	1239-1528	1.000000	90.0
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4137	5585	1238-1527	1.000000	90.0
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	CCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.3	58.1	5.3	16.9	0.358907	4137	5585	1248-1537	1.000000	89.1
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.536858	4137	5585	1225-1514	1.000000	87.7
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	GTGTAAAAGATGCTAGCCGC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.3	58.1	13.3	16.9	0.815394	4137	5585	1264-1553	1.000000	85.5
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TTCATTTCCACTCCCAAGCA	CCGGATTTGAACTGGGGAAA	20	20	45.0	50.0	57.3	58.1	0.0	0.0	0.755903	25033	26085	1075-1165	1.000000	86.0
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCATTTCCACTCCCAAGCAT	CCGGATTTGAACTGGGGAAA	20	20	45.0	50.0	57.1	58.1	0.0	0.0	0.987543	25033	26085	1074-1164	1.000000	84.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TTCATTTCCACTCCCAAGCA	AAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	57.3	58.4	0.0	14.6	1.029266	25033	26085	1057-1147	1.000000	83.8
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCATTTCCACTCCCAAGCAT	CCGGATTTGAACTGGGGAAA	21	20	42.9	50.0	57.8	58.1	0.0	0.0	1.256081	25033	26085	1075-1165	1.000000	82.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCATTTCCACTCCCAAGCAT	AAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	57.1	58.4	0.0	14.6	1.260906	25033	26085	1056-1146	1.000000	81.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	26167	26952	841-865	1.000000	88.8
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGTGTCGCCTGATAACCAAA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.335274	26167	26952	822-846	1.000000	88.1
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTAGATCGTTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	58.3	58.2	14.3	0.0	0.406116	26167	26952	895-919	1.000000	87.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	26167	26952	902-926	1.000000	87.1
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	26167	26952	859-883	1.000000	86.6
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.716274	28018	28401	408-458	1.000000	86.3
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.717825	28018	28401	407-457	1.000000	86.3
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	28018	28401	429-479	1.000000	85.4
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	28018	28401	428-478	1.000000	85.2
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	20	20	50.0	50.0	58.5	57.5	7.1	0.0	0.998018	28018	28401	424-474	1.000000	84.0
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	28617	29180	613-672	1.000000	90.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	28617	29180	611-670	1.000000	90.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	28617	29180	667-726	1.000000	90.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	28617	29180	592-651	1.000000	90.3
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.209464	28617	29180	590-649	1.000000	90.3
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CCGGTACATCATTATGGATTGGA	AATCGAACCCGCATCTTCTC	23	20	43.5	50.0	58.1	58.1	0.0	0.0	3.153246	33597	33974	499-523	1.000000	66.8
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CCGGTACATCATTATGGATTGGA	GAATCGAACCCGCATCTTCT	23	20	43.5	50.0	58.1	58.1	0.0	0.0	3.153246	33597	33974	500-524	1.000000	66.8
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TCCGGTACATCATTATGGATTGG	AATCGAACCCGCATCTTCTC	23	20	43.5	50.0	58.1	58.1	0.0	0.0	3.153246	33597	33974	500-524	1.000000	66.8
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TCCGGTACATCATTATGGATTGG	GAATCGAACCCGCATCTTCT	23	20	43.5	50.0	58.1	58.1	0.0	0.0	3.153246	33597	33974	501-525	1.000000	66.8
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CCGGTACATCATTATGGATTGG	AATCGAACCCGCATCTTCTC	22	20	45.5	50.0	56.7	58.1	0.0	0.0	3.356024	33597	33974	499-523	1.000000	65.2
psaA-ycf3_p1	psaA-ycf3	1	CGGGGTCTCTCTCTACCAAA	AAGATCACAAGGCGTTTCGA	20	20	55.0	45.0	58.2	57.8	0.0	0.0	0.315166	39331	40007	743-771	1.000000	88.3
psaA-ycf3_p2	psaA-ycf3	2	CGGGGTCTCTCTCTACCAAA	AGATCACAAGGCGTTTCGAA	20	20	55.0	45.0	58.2	57.8	0.0	0.0	0.315166	39331	40007	742-770	1.000000	88.3
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	39331	40007	768-796	1.000000	88.2
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	39331	40007	767-795	1.000000	88.2
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCGGGTAAT	20	20	50.0	50.0	57.9	58.3	4.1	18.7	0.419280	39331	40007	812-840	1.000000	86.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	44266	44980	551-795	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	44266	44980	582-826	1.000000	90.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	44266	44980	559-803	1.000000	90.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	44266	44980	590-834	1.000000	90.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AACAGATGGAGAAAAGCCCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.178239	44266	44980	608-852	0.900000	86.6
rbcL-accD_p1	rbcL-accD	1	GCCGTAGCTAATCGAGTAGC	TACTGAAACTCCCGCTTTCG	20	20	55.0	50.0	58.0	57.9	31.7	0.0	0.108125	53375	54092	1423-1479	0.850000	85.0
rbcL-accD_p2	rbcL-accD	2	GGCTTCAGGGGGTATTCATG	TACTGAAACTCCCGCTTTCG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.087665	53375	54092	1541-1597	0.850000	84.8
rbcL-accD_p3	rbcL-accD	3	GCCGTAGCTAATCGAGTAGC	ACTCGCCCTATGACTATCCC	20	20	55.0	55.0	58.0	58.1	31.7	0.0	0.107470	53375	54092	1116-1172	0.750000	80.7
rbcL-accD_p4	rbcL-accD	4	GGCTTCAGGGGGTATTCATG	ACTCGCCCTATGACTATCCC	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.087009	53375	54092	1234-1290	0.750000	80.5
rbcL-accD_p5	rbcL-accD	5	GAGGCAGCTAAATGGAGTCC	ACTCGCCCTATGACTATCCC	20	20	55.0	55.0	58.0	58.1	0.7	0.0	0.113475	53375	54092	1026-1076	0.250000	56.2
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	TCAAAGCTGCTAGTAGAAAACCA	20	23	50.0	39.1	57.8	58.0	24.9	12.5	3.188084	60612	61682	1132-1165	0.950000	63.4
psbE-petL_p2	psbE-petL	2	GTTCTCCTGTGCTTCCAGAC	TCAAAGCTGCTAGTAGAAAACCA	20	23	55.0	39.1	58.2	58.0	0.9	12.5	3.229672	60612	61682	1127-1160	0.950000	63.1
psbE-petL_p3	psbE-petL	3	TCTCCTGTGCTTCCAGACAT	TCAAAGCTGCTAGTAGAAAACCA	20	23	50.0	39.1	58.4	58.0	0.9	12.5	3.382419	60612	61682	1125-1158	0.950000	61.9
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GTCAAAGCTGCTAGTAGAAAACC	20	23	50.0	43.5	57.8	57.7	24.9	12.5	3.440771	60612	61682	1133-1166	0.950000	61.4
psbE-petL_p5	psbE-petL	5	GTTCTCCTGTGCTTCCAGAC	GTCAAAGCTGCTAGTAGAAAACC	20	23	55.0	43.5	58.2	57.7	0.9	12.5	3.482359	60612	61682	1128-1161	0.950000	61.1
rps19_p1	rps19	1	ATGCTCGAAACGGCATAAGT	ACTATGGGGGTGGTGAGAAA	20	20	45.0	50.0	58.0	57.9	8.0	0.0	0.077922	79662	79880	1112-1837	1.000000	91.4
rps19_p2	rps19	2	GCTTTGTGAGCGGACATAGA	AAAAGTTTGGGTAGAGCCGG	20	20	50.0	50.0	58.0	58.1	11.1	0.0	0.104508	79662	79880	419-845	0.850000	85.2
rps19_p3	rps19	3	GCTTTGTGAGCGGACATAGA	TTGATTCTTCGTCGCCGTAG	20	20	50.0	50.0	58.0	58.1	11.1	0.0	0.106001	79662	79880	221-647	0.850000	85.2
rps19_p4	rps19	4	GCTTTGTGAGCGGACATAGA	ACTATGGGGGTGGTGAGAAA	20	20	50.0	50.0	58.0	57.9	11.1	0.0	0.063892	79662	79880	1032-1757	0.850000	83.3
rps19_p5	rps19	5	TTTTCCTATAGCGCGCACTT	ACTATGGGGGTGGTGAGAAA	20	20	45.0	50.0	58.0	57.9	35.1	0.0	0.072289	79662	79880	1006-1731	0.700000	78.9
ndhF-rpl32_p1	ndhF-rpl32	1	CTCCAACGCGATTAGTCACA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.017031	106650	107414	1833-1983	0.950000	89.9
ndhF-rpl32_p2	ndhF-rpl32	2	CTCCAACGCGATTAGTCACA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.018402	106650	107414	1834-1984	0.950000	89.9
ndhF-rpl32_p3	ndhF-rpl32	3	CTCCAACGCGATTAGTCACA	TTAGAAGATGACATGCCGCC	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.055642	106650	107414	1897-2047	0.950000	89.6
ndhF-rpl32_p4	ndhF-rpl32	4	CTCCAACGCGATTAGTCACA	TAGCGGCTTGTTCGAAATCA	20	20	50.0	45.0	58.0	57.9	15.9	8.3	0.096702	106650	107414	1811-1961	0.950000	89.2
ndhF-rpl32_p5	ndhF-rpl32	5	CTCCAACGCGATTAGTCACA	TCGAACCGAGATGCTTTAGC	20	20	50.0	50.0	58.0	58.1	15.9	0.0	0.086630	106650	107414	1990-2019	0.750000	79.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCATTCAAAGCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	9.5	0.0	0.182194	107574	108035	567-593	1.000000	89.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCATTCAAAGCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	9.5	0.0	0.183564	107574	108035	568-594	1.000000	89.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGCAGCATTCAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.8	57.9	9.5	0.0	0.298641	107574	108035	577-603	1.000000	88.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGCAGCATTCAAAGCTTTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.8	57.8	9.5	0.0	0.370658	107574	108035	576-602	1.000000	87.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCATTCAAAGCTTTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.8	57.8	9.5	0.0	0.370658	107574	108035	575-601	1.000000	87.8
rps15-ycf1_p1	rps15-ycf1	1	TCCCAACGCGTAACATAAGG	CTAAAAAGGGCGGGACACTT	20	20	50.0	50.0	58.0	58.1	14.5	0.0	0.107299	117395	117761	1447-1463	1.000000	88.4
rps15-ycf1_p2	rps15-ycf1	2	TCCCAACGCGTAACATAAGG	AACCCCTTCGTCTGTGTCTA	20	20	50.0	50.0	58.0	58.0	14.5	0.0	0.018307	117395	117761	1464-1477	0.850000	82.9
rps15-ycf1_p3	rps15-ycf1	3	TCCCAACGCGTAACATAAGG	ACCCCTTCGTCTGTGTCTAA	20	20	50.0	50.0	58.0	58.0	14.5	0.0	0.018307	117395	117761	1463-1476	0.850000	82.9
rps15-ycf1_p4	rps15-ycf1	4	AACACCATGCATTGATGGGT	AACCCCTTCGTCTGTGTCTA	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.063993	117395	117761	1327-1340	0.800000	80.7
rps15-ycf1_p5	rps15-ycf1	5	AACACCATGCATTGATGGGT	ACCCCTTCGTCTGTGTCTAA	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.063993	117395	117761	1326-1339	0.800000	80.7
ycf1_p1	ycf1	1	CGTCGAAAATCCGGTTGTTG	CTTCCCGTGGACAAATGCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.162743	121338	121457	613-622	0.600000	71.9
ycf1_p2	ycf1	2	CGTCGAAAATCCGGTTGTTG	TTACGCACCCACCTAGTTTG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.227644	121338	121457	910-919	0.500000	66.7
ycf1_p3	ycf1	3	CGTCGAAAATCCGGTTGTTG	TGTTTTACGCACCCACCTAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.227644	121338	121457	914-923	0.500000	66.6
ycf1_p4	ycf1	4	CGTCGAAAATCCGGTTGTTG	CACCCACCTAGTTTGTCCAC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.162583	121338	121457	914-914	0.300000	52.5
ycf1_p5	ycf1	5	ATCCTCGGACCATTCAGGAA	CACCCACCTAGTTTGTCCAC	20	20	50.0	55.0	58.1	58.1	3.3	0.0	0.228216	121338	121457	1362-1362	0.300000	52.0