Hotspot

trnI-CAU

IRa · 156120-156188 · 69 bp

Back to Urophysa

Pi 0.087
MarkerSeek score 72.00
Primer available yes
Label trnI-CAU

Feature Metadata

label_name
trnI-CAU
feature_type
tRNA
region
IRa
length_bp
69
variable_sites
6
pi
0.087
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU_p1 CTTGCGGTAATGATGCCTCT TAGGAAGAAGCCCCCTCAAA 50.0 50.0 58.0 57.9 1091-1102 bp 1.0 81.0
trnI-CAU_p2 CTTGCGGTAATGATGCCTCT GTCACTGCGTCCTCTTTCAT 50.0 50.0 58.0 57.9 765-776 bp 1.0 81.0
trnI-CAU_p3 AGAAGAGTTCGACCCAATGC TAGGAAGAAGCCCCCTCAAA 50.0 50.0 57.9 57.9 775-780 bp 1.0 80.6
trnI-CAU_p4 GAAGAGTTCGACCCAATGCT TAGGAAGAAGCCCCCTCAAA 50.0 50.0 57.9 57.9 774-779 bp 1.0 80.6
trnI-CAU_p5 GGGTCCCATTCTTCTACCCT TAGGAAGAAGCCCCCTCAAA 55.0 50.0 58.1 57.9 840-845 bp 1.0 80.4
trnI-CAU_p1 TAGGAAGAAGCCCCCTCAAA CTTGCGGTAATGATGCCTCT 50.0 50.0 57.9 58.0 1091-1102 bp 1.0 81.1
trnI-CAU_p2 GTCACTGCGTCCTCTTTCAT CTTGCGGTAATGATGCCTCT 50.0 50.0 57.9 58.0 765-776 bp 1.0 81.1
trnI-CAU_p3 TAGGAAGAAGCCCCCTCAAA AGAAGAGTTCGACCCAATGC 50.0 50.0 57.9 57.9 775-780 bp 1.0 80.6
trnI-CAU_p4 TAGGAAGAAGCCCCCTCAAA GAAGAGTTCGACCCAATGCT 50.0 50.0 57.9 57.9 774-779 bp 1.0 80.6
trnI-CAU_p5 TAGGAAGAAGCCCCCTCAAA GGGTCCCATTCTTCTACCCT 50.0 55.0 57.9 58.1 840-845 bp 1.0 80.4