Hotspot

rps4

LSC · 47736-48317 · 582 bp

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Pi 0.0292

MarkerSeek score 68.80

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 582
variable_sites: 17
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	GAAAAATCCAGACCAAGCGC	TGGATTTAGCTTCGTTTATCGT	50.0	36.4	58.0	55.9	766 bp	0.5	27.2
rps4_p2	GAAAAATCCAGACCAAGCGC	GCTTCGTTTATCGTTTAGTTCAGT	50.0	37.5	58.0	57.9	758 bp	0.5	26.7
rps4_p3	GAAAAATCCAGACCAAGCGC	TGGATTTAGCTTCGTTTATCGTT	50.0	34.8	58.0	56.6	766 bp	0.5	24.8
rps4_p4	CGCCTTCCCTTTACCTTAGG	TGGATTTAGCTTCGTTTATCGT	55.0	36.4	57.7	55.9	749 bp	0.5	24.7
rps4_p5	GAAAAATCCAGACCAAGCGC	AGCTTCGTTTATCGTTTAGTTCAG	50.0	37.5	58.0	57.6	759 bp	0.5	24.7