Hotspot

rrn4.5-rrn23

IRa · 134679-134776 · 98 bp

Back to Urophysa

Pi 0.0306

MarkerSeek score 70.10

Primer available yes

Label rrn4.5-rrn23

Feature Metadata

label_name: rrn4.5-rrn23
feature_type: igs
region: IRa
length_bp: 98
variable_sites: 3
pi: 0.0306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn23_p1	TCACTGCACTTCCACTTGAC	AAGTAGTAAGCCCACCCCAA	50.0	50.0	58.0	58.2	194 bp	1.0	78.9
rrn4.5-rrn23_p2	TGGTCTGTTAGGATGCCTCA	AAGTAGTAAGCCCACCCCAA	50.0	50.0	58.0	58.2	224 bp	1.0	78.7
rrn4.5-rrn23_p3	CTGTTAGGATGCCTCAGCTG	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.0	58.2	220 bp	1.0	78.7
rrn4.5-rrn23_p4	GTCTGTTAGGATGCCTCAGC	AAGTAGTAAGCCCACCCCAA	55.0	50.0	58.1	58.2	222 bp	1.0	78.7
rrn4.5-rrn23_p5	TCACTGCACTTCCACTTGAC	TAGTAAGCCCACCCCAAGAT	50.0	50.0	58.0	57.7	191 bp	1.0	78.6