Hotspot

rps14-psaB

LSC · 39263-39391 · 129 bp

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Pi 0.0431

MarkerSeek score 80.20

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 5
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CACTCACCGACGAAACTCTT	GTGGCTCTATCCATTGTGCA	50.0	50.0	57.9	58.0	345-358 bp	1.0	82.7
rps14-psaB_p2	CACTCACCGACGAAACTCTT	GCAGCTTTCTTGATTGCCTC	50.0	50.0	57.9	57.7	273-286 bp	1.0	81.9
rps14-psaB_p3	TTTGTCACTCACCGACGAAA	GTGGCTCTATCCATTGTGCA	45.0	50.0	57.7	58.0	350-363 bp	1.0	81.3
rps14-psaB_p4	CACTCACCGACGAAACTCTT	AGTGGCTCTATCCATTGTGC	50.0	50.0	57.9	57.7	346-359 bp	1.0	80.3
rps14-psaB_p5	TTGTCACTCACCGACGAAAC	GTGGCTCTATCCATTGTGCA	50.0	50.0	58.4	58.0	349-362 bp	1.0	80.2