Hotspot

trnS-GCU-trnG-UCC

LSC · 8558-9310 · 753 bp

Back to Urophysa

Pi 0.0135

MarkerSeek score 68.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 10
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AACCGAAAGAACCCTTAACT	50.0	40.0	58.0	54.5	849-860 bp	1.0	54.0
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AACCGAAAGAACCCTTAACT	50.0	40.0	58.0	54.5	848-859 bp	1.0	54.0
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	811-822 bp	1.0	53.9
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	810-821 bp	1.0	53.9
trnS-GCU-trnG-UCC_p5	TCAGAAGAGGCGGAAAGAGA	AACCGAAAGAACCCTTAACT	50.0	40.0	58.1	54.5	912-923 bp	1.0	53.3