primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	AACCGAAAGAACCCTTAACT	20	20	50.0	40.0	58.0	54.5	0.0	0.0	3.519820	8558	9310	849-860	1.000000	54.0
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	AACCGAAAGAACCCTTAACT	20	20	50.0	40.0	58.0	54.5	0.0	0.0	3.521208	8558	9310	848-859	1.000000	54.0
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8558	9310	811-822	1.000000	53.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8558	9310	810-821	1.000000	53.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TCAGAAGAGGCGGAAAGAGA	AACCGAAAGAACCCTTAACT	20	20	50.0	40.0	58.1	54.5	0.0	0.0	3.590506	8558	9310	912-923	1.000000	53.3
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AAATGAAGGGCGTCCATTGT	GAACAGACGGAGCGGTTTAT	20	20	45.0	50.0	58.1	58.0	9.7	0.0	0.082615	10306	10441	244-256	1.000000	84.1
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	AGGGCGTCCATTGTCTAATG	GAACAGACGGAGCGGTTTAT	20	20	50.0	50.0	57.7	58.0	9.7	0.0	0.338034	10306	10441	238-250	1.000000	82.2
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AAATGAAGGGCGTCCATTGT	CGAAGAAGCGGAAGTCCTTT	20	20	45.0	50.0	58.1	58.2	9.7	0.0	0.279620	10306	10441	281-293	1.000000	81.9
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	AAATGAAGGGCGTCCATTGT	CGACCAAGACATTCACCGAA	20	20	45.0	50.0	58.1	58.2	9.7	0.0	0.285889	10306	10441	297-309	1.000000	81.7
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	AAATGAAGGGCGTCCATTGT	TTCGACCAAGACATTCACCG	20	20	45.0	50.0	58.1	58.2	9.7	0.0	0.285889	10306	10441	299-311	1.000000	81.6
rps14-psaB_p1	rps14-psaB	1	CACTCACCGACGAAACTCTT	GTGGCTCTATCCATTGTGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.178350	39263	39391	345-358	1.000000	82.7
rps14-psaB_p2	rps14-psaB	2	CACTCACCGACGAAACTCTT	GCAGCTTTCTTGATTGCCTC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.410584	39263	39391	273-286	1.000000	81.9
rps14-psaB_p3	rps14-psaB	3	TTTGTCACTCACCGACGAAA	GTGGCTCTATCCATTGTGCA	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.339517	39263	39391	350-363	1.000000	81.3
rps14-psaB_p4	rps14-psaB	4	CACTCACCGACGAAACTCTT	AGTGGCTCTATCCATTGTGC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.474241	39263	39391	346-359	1.000000	80.3
rps14-psaB_p5	rps14-psaB	5	TTGTCACTCACCGACGAAAC	GTGGCTCTATCCATTGTGCA	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.479575	39263	39391	349-362	1.000000	80.2
rps4_p1	rps4	1	GAAAAATCCAGACCAAGCGC	TGGATTTAGCTTCGTTTATCGT	20	22	50.0	36.4	58.0	55.9	0.0	0.0	4.097306	47736	48317	766-766	0.500000	27.2
rps4_p2	rps4	2	GAAAAATCCAGACCAAGCGC	GCTTCGTTTATCGTTTAGTTCAGT	20	24	50.0	37.5	58.0	57.9	0.0	0.0	4.166892	47736	48317	758-758	0.500000	26.7
rps4_p3	rps4	3	GAAAAATCCAGACCAAGCGC	TGGATTTAGCTTCGTTTATCGTT	20	23	50.0	34.8	58.0	56.6	0.0	0.0	4.393946	47736	48317	766-766	0.500000	24.8
rps4_p4	rps4	4	CGCCTTCCCTTTACCTTAGG	TGGATTTAGCTTCGTTTATCGT	20	22	55.0	36.4	57.7	55.9	0.0	0.0	4.407118	47736	48317	749-749	0.500000	24.7
rps4_p5	rps4	5	GAAAAATCCAGACCAAGCGC	AGCTTCGTTTATCGTTTAGTTCAG	20	24	50.0	37.5	58.0	57.6	0.0	0.0	4.408374	47736	48317	759-759	0.500000	24.7
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TAGTCCCGGTAAAACACCCA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.3	57.9	0.0	0.0	0.405678	48318	48654	462-463	1.000000	77.8
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	TAGTCCCGGTAAAACACCCA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.513431	48318	48654	430-431	1.000000	77.0
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	TAGTCCCGGTAAAACACCCA	CAGAGGTTAGAGCATCGCAT	20	20	50.0	50.0	58.3	57.5	0.0	0.0	0.795774	48318	48654	460-461	1.000000	74.7
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TAGTCCCGGTAAAACACCCA	TCAGAGGTTAGAGCATCGCA	20	20	50.0	50.0	58.3	58.5	0.0	0.0	0.798608	48318	48654	461-462	1.000000	74.7
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TAGTCCCGGTAAAACACCCA	AAGTCCTATGCACAGCACAA	20	20	50.0	45.0	58.3	57.4	0.0	0.0	0.839771	48318	48654	506-507	1.000000	74.2
trnL-UAA_p1	trnL-UAA	1	TGGCATCCTAACGAGATCCT	TGGAATGGAACCGCTGAAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.099811	50064	50630	683-683	0.500000	59.2
trnL-UAA_p2	trnL-UAA	2	TGGCATCCTAACGAGATCCT	TTGGAATGGAACCGCTGAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.099811	50064	50630	684-684	0.500000	59.2
trnL-UAA_p3	trnL-UAA	3	CCATGTAAACGGCTCCAGAA	TGGAATGGAACCGCTGAAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.119657	50064	50630	726-726	0.500000	59.0
trnL-UAA_p4	trnL-UAA	4	CCATGTAAACGGCTCCAGAA	TTGGAATGGAACCGCTGAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.119657	50064	50630	727-727	0.500000	59.0
trnL-UAA_p5	trnL-UAA	5	TTCCATGTAAACGGCTCCAG	TGGAATGGAACCGCTGAAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.119657	50064	50630	728-728	0.500000	59.0
ycf4-cemA_p1	ycf4-cemA	1	CGTTCCCTTGACTCGTACTG	GAGAAATCCACCAGGGCAAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.098103	63893	64689	963-973	1.000000	81.9
ycf4-cemA_p2	ycf4-cemA	2	TGCGCGTACCAATTGAAGTA	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.9	58.1	8.4	0.0	0.158370	63893	64689	886-896	1.000000	81.6
ycf4-cemA_p3	ycf4-cemA	3	ACAAAAAGCTGCGGAATTGG	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.230011	63893	64689	915-925	1.000000	80.9
ycf4-cemA_p4	ycf4-cemA	4	CGTTCCCTTGACTCGTACTG	AAGGGGTTTTAGCGCTTTCT	20	20	55.0	45.0	58.0	57.7	0.0	18.7	0.309436	63893	64689	920-930	1.000000	80.3
ycf4-cemA_p5	ycf4-cemA	5	CGTTCCCTTGACTCGTACTG	AGGGGTTTTAGCGCTTTCTT	20	20	55.0	45.0	58.0	57.7	0.0	18.7	0.309436	63893	64689	919-929	1.000000	80.3
trnI-CAU_p1	trnI-CAU	1	CTTGCGGTAATGATGCCTCT	TAGGAAGAAGCCCCCTCAAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.094705	89147	89215	1091-1102	1.000000	81.0
trnI-CAU_p2	trnI-CAU	2	CTTGCGGTAATGATGCCTCT	GTCACTGCGTCCTCTTTCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.127013	89147	89215	765-776	1.000000	81.0
trnI-CAU_p3	trnI-CAU	3	AGAAGAGTTCGACCCAATGC	TAGGAAGAAGCCCCCTCAAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.154351	89147	89215	775-780	1.000000	80.6
trnI-CAU_p4	trnI-CAU	4	GAAGAGTTCGACCCAATGCT	TAGGAAGAAGCCCCCTCAAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.154351	89147	89215	774-779	1.000000	80.6
trnI-CAU_p5	trnI-CAU	5	GGGTCCCATTCTTCTACCCT	TAGGAAGAAGCCCCCTCAAA	20	20	55.0	50.0	58.1	57.9	1.8	0.0	0.159048	89147	89215	840-845	1.000000	80.4
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	20	20	45.0	50.0	57.7	57.9	16.5	0.0	0.380474	101702	103559	2015-2015	1.000000	78.3
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	101702	103559	1954-1954	1.000000	78.0
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	101702	103559	1958-1958	1.000000	78.0
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	101702	103559	1953-1953	1.000000	78.0
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	101702	103559	1956-1956	1.000000	77.0
rrn23-rrn4.5_p1	rrn23-rrn4.5	1	AAGTAGTAAGCCCACCCCAA	TCACTGCACTTCCACTTGAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.296419	110559	110656	194-194	1.000000	78.9
rrn23-rrn4.5_p2	rrn23-rrn4.5	2	AAGTAGTAAGCCCACCCCAA	TGGTCTGTTAGGATGCCTCA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.298768	110559	110656	224-224	1.000000	78.7
rrn23-rrn4.5_p3	rrn23-rrn4.5	3	AAGTAGTAAGCCCACCCCAA	CTGTTAGGATGCCTCAGCTG	20	20	50.0	55.0	58.2	58.0	0.0	7.6	0.299150	110559	110656	220-220	1.000000	78.7
rrn23-rrn4.5_p4	rrn23-rrn4.5	4	AAGTAGTAAGCCCACCCCAA	GTCTGTTAGGATGCCTCAGC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.303233	110559	110656	222-222	1.000000	78.7
rrn23-rrn4.5_p5	rrn23-rrn4.5	5	TAGTAAGCCCACCCCAAGAT	TCACTGCACTTCCACTTGAC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.326749	110559	110656	191-191	1.000000	78.6
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	GGGGGCGTATCTCCTCTTAT	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.062351	112457	113537	1505-1585	1.000000	81.2
ycf1_p2	ycf1	2	CAATTCCCGTTCTCAACCCA	GGGGGCGTATCTCCTCTTAT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.155733	112457	113537	1464-1544	1.000000	80.3
ycf1_p3	ycf1	3	ATCTTTGATGCACTGCGAGT	GGGGGCGTATCTCCTCTTAT	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.165026	112457	113537	1822-1901	1.000000	80.2
ycf1_p4	ycf1	4	ATTCCCGTTCTCAACCCATG	GGGGGCGTATCTCCTCTTAT	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.188791	112457	113537	1462-1542	1.000000	80.1
ycf1_p5	ycf1	5	GCTTCCTTCGTAACTACCGG	GGGGGCGTATCTCCTCTTAT	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.141425	112457	113537	1425-1425	0.500000	58.9
rrn4.5-rrn23_p1	rrn4.5-rrn23	1	TCACTGCACTTCCACTTGAC	AAGTAGTAAGCCCACCCCAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.296419	134679	134776	194-194	1.000000	78.9
rrn4.5-rrn23_p2	rrn4.5-rrn23	2	TGGTCTGTTAGGATGCCTCA	AAGTAGTAAGCCCACCCCAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.298768	134679	134776	224-224	1.000000	78.7
rrn4.5-rrn23_p3	rrn4.5-rrn23	3	CTGTTAGGATGCCTCAGCTG	AAGTAGTAAGCCCACCCCAA	20	20	55.0	50.0	58.0	58.2	7.6	0.0	0.299150	134679	134776	220-220	1.000000	78.7
rrn4.5-rrn23_p4	rrn4.5-rrn23	4	GTCTGTTAGGATGCCTCAGC	AAGTAGTAAGCCCACCCCAA	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.303233	134679	134776	222-222	1.000000	78.7
rrn4.5-rrn23_p5	rrn4.5-rrn23	5	TCACTGCACTTCCACTTGAC	TAGTAAGCCCACCCCAAGAT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.326749	134679	134776	191-191	1.000000	78.6
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.9	57.7	0.0	16.5	0.380474	141776	143633	2015-2015	1.000000	78.3
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	141776	143633	1954-1954	1.000000	78.0
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	141776	143633	1958-1958	1.000000	78.0
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	141776	143633	1953-1953	1.000000	78.0
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	141776	143633	1956-1956	1.000000	77.0
ycf2_p1	ycf2	1	GCTGTAGGAGATTTTGTTTGGG	GTTCTTGCAGAGTGGAACCA	22	20	45.5	50.0	57.6	58.0	0.0	0.0	2.473520	149484	149603	219-219	1.000000	64.2
ycf2_p2	ycf2	2	GCTGTAGGAGATTTTGTTTGGG	TTCGGAGTTCTTGCAGAGTG	22	20	45.5	50.0	57.6	57.8	0.0	0.0	2.595693	149484	149603	225-225	1.000000	63.1
ycf2_p3	ycf2	3	GCTGTAGGAGATTTTGTTTGGG	TCTTGCAGAGTGGAACCATG	22	20	45.5	50.0	57.6	57.8	0.0	0.0	2.630433	149484	149603	217-217	1.000000	63.0
ycf2_p4	ycf2	4	GCTGTAGGAGATTTTGTTTGGG	GAGTGGAACCATGCAGTACC	22	20	45.5	55.0	57.6	58.3	0.0	0.0	2.701772	149484	149603	210-210	1.000000	62.6
ycf2_p5	ycf2	5	GCTGTAGGAGATTTTGTTTGGG	AGTTCTTGCAGAGTGGAACC	22	20	45.5	50.0	57.6	57.7	0.0	11.8	2.703262	149484	149603	220-220	1.000000	62.4
trnI-CAU_p1	trnI-CAU	1	TAGGAAGAAGCCCCCTCAAA	CTTGCGGTAATGATGCCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.094705	156120	156188	1091-1102	1.000000	81.1
trnI-CAU_p2	trnI-CAU	2	GTCACTGCGTCCTCTTTCAT	CTTGCGGTAATGATGCCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.127013	156120	156188	765-776	1.000000	81.1
trnI-CAU_p3	trnI-CAU	3	TAGGAAGAAGCCCCCTCAAA	AGAAGAGTTCGACCCAATGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.154351	156120	156188	775-780	1.000000	80.6
trnI-CAU_p4	trnI-CAU	4	TAGGAAGAAGCCCCCTCAAA	GAAGAGTTCGACCCAATGCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.154351	156120	156188	774-779	1.000000	80.6
trnI-CAU_p5	trnI-CAU	5	TAGGAAGAAGCCCCCTCAAA	GGGTCCCATTCTTCTACCCT	20	20	50.0	55.0	57.9	58.1	0.0	1.8	0.159048	156120	156188	840-845	1.000000	80.4