Hotspot

rpoC2

LSC · 25865-30472 · 4608 bp

Back to Urochloa

Pi 0.0098

MarkerSeek score 45.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4608
variable_sites: 83
pi: 0.0098
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TCGGACCCTAGAAGAAGAGT	TCATATTCGTCCTCTAGAGTCCT	50.0	43.5	56.8	57.4	189-192 bp	1.0	47.3
rpoC2_p2	TCGGACCCTAGAAGAAGAGT	TCATATTCGTCCTCTAGAGTCC	50.0	45.5	56.8	56.0	189-192 bp	1.0	45.5
rpoC2_p3	TCGGACCCTAGAAGAAGAGT	CATATTCGTCCTCTAGAGTCCT	50.0	45.5	56.8	56.0	188-191 bp	1.0	45.5
rpoC2_p4	ATCGGACCCTAGAAGAAGAGT	TCATATTCGTCCTCTAGAGTCCT	47.6	43.5	57.3	57.4	190-193 bp	1.0	45.5
rpoC2_p5	AGAGGAAGAATATCGGACCCT	TCATATTCGTCCTCTAGAGTCCT	47.6	43.5	57.4	57.4	201-204 bp	1.0	45.2