Hotspot

rpl16

LSC · 78303-79669 · 1367 bp

Back to Urochloa

Pi 0.0215

MarkerSeek score 60.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1367
variable_sites: 43
pi: 0.0215
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	TGCATCAAAAAGGGCAGACT	55.0	45.0	57.9	58.0	1758-1810 bp	1.0	84.2
rpl16_p2	TCGGATCTTTCTAGGGGCAT	TGCATCAAAAAGGGCAGACT	50.0	45.0	57.9	58.0	1837-1889 bp	1.0	83.8
rpl16_p3	TTCGGATCTTTCTAGGGGCA	TGCATCAAAAAGGGCAGACT	50.0	45.0	58.1	58.0	1838-1890 bp	1.0	83.6
rpl16_p4	CTCAATTCTTCGGCAATCGC	TGCATCAAAAAGGGCAGACT	50.0	45.0	57.9	58.0	1990-2042 bp	1.0	83.5
rpl16_p5	GATTACCAGCGGCTCCTATG	CCATTCCTTTTGGTTCGCAC	55.0	50.0	57.9	57.9	2254-2306 bp	1.0	82.9