Hotspot

ccsA-ndhD

SSC · 108586-108795 · 210 bp

Back to Urochloa

Pi 0.0437

MarkerSeek score 69.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 210
variable_sites: 18
pi: 0.0437
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAACTCTTTTGTGCGGATC	ATTCTGGACCACGGGAACTA	50.0	50.0	58.0	58.1	870-876 bp	1.0	83.4
ccsA-ndhD_p2	GCAACTCTTTTGTGCGGATC	TCTGGACCACGGGAACTATT	50.0	50.0	58.0	58.1	868-874 bp	1.0	83.4
ccsA-ndhD_p3	GCAACTCTTTTGTGCGGATC	TTCTGGACCACGGGAACTAT	50.0	50.0	58.0	58.1	869-875 bp	1.0	83.4
ccsA-ndhD_p4	AAAGCACCTCTTTTCTCGCA	TCTGGACCACGGGAACTATT	45.0	50.0	58.0	58.1	715-716 bp	0.75	70.7
ccsA-ndhD_p5	AAAGCACCTCTTTTCTCGCA	TTCTGGACCACGGGAACTAT	45.0	50.0	58.0	58.1	716-717 bp	0.75	70.7