Hotspot

rps11-rpl36

LSC · 76488-76659 · 172 bp

Back to Urochloa

Pi 0.03

MarkerSeek score 66.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 9
pi: 0.03
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGCAAACCAATACGCACTTT	GCGTGGACGAATTAGAGTCA	45.0	50.0	58.0	57.7	284-288 bp	1.0	80.5
rps11-rpl36_p2	CTACGCAAACCAATACGCAC	GCGTGGACGAATTAGAGTCA	50.0	50.0	57.8	57.7	287-291 bp	1.0	79.6
rps11-rpl36_p3	TACGCAAACCAATACGCACT	GCGTGGACGAATTAGAGTCA	45.0	50.0	58.2	57.7	286-290 bp	1.0	79.3
rps11-rpl36_p4	CGCAAACCAATACGCACTTT	CAGGCGTGGACGAATTAGAG	45.0	55.0	58.0	58.4	287-291 bp	1.0	79.2
rps11-rpl36_p5	TCTACGTGCACTCTTCCGTA	GCGTGGACGAATTAGAGTCA	50.0	50.0	58.2	57.7	324-328 bp	1.0	79.0