Hotspot

rbcL-psaI

LSC · 57103-57906 · 804 bp

Back to Urochloa

Pi 0.0286

MarkerSeek score 67.60

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 40
pi: 0.0286
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1174-1197 bp	1.0	86.1
rbcL-psaI_p2	TTTTTCACTCAGGACTGGGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1218-1241 bp	1.0	85.8
rbcL-psaI_p3	GTTATACCGGTGGCTTCAGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1185-1208 bp	1.0	85.7
rbcL-psaI_p4	GGCTTCAGGGGGTATTCATG	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1708-1730 bp	1.0	84.3
rbcL-psaI_p5	TTTTTCACTCAGGACTGGGC	CCAAGATAACTGGAAGCCCC	50.0	55.0	58.0	57.9	1752-1774 bp	1.0	84.1