Hotspot

ndhC-trnV-UAC

LSC · 51013-51948 · 936 bp

Back to Urochloa

Pi 0.0144

MarkerSeek score 65.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 936
variable_sites: 25
pi: 0.0144
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACAGGGGCTAAAAGTCCAGA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	1059-1065 bp	1.0	79.7
ndhC-trnV-UAC_p2	ACAGGGGCTAAAAGTCCAGA	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	1060-1066 bp	1.0	77.8
ndhC-trnV-UAC_p3	AACAGGGGCTAAAAGTCCAG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.4	57.9	1060-1066 bp	1.0	77.0
ndhC-trnV-UAC_p4	TCCTTCACTAACAGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.4	57.9	1069-1075 bp	1.0	76.6
ndhC-trnV-UAC_p5	TGGTCCTTCACTAACAGGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.6	57.9	1072-1078 bp	1.0	76.6