Hotspot

ycf3-trnS-GGA

LSC · 45150-45626 · 477 bp

Back to Urochloa

Pi 0.0238

MarkerSeek score 70.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 21
pi: 0.0238
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGGCTACAATCGAAGAGGTC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.0	58.2	617-623 bp	1.0	74.8
ycf3-trnS-GGA_p2	TGGCTACAATCGAAGAGGTC	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.0	57.8	567-573 bp	1.0	74.8
ycf3-trnS-GGA_p3	TTGGCTACAATCGAAGAGGTC	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.8	58.2	618-624 bp	1.0	72.5
ycf3-trnS-GGA_p4	GGCTACAATCGAAGAGGTCT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	56.7	58.2	616-622 bp	1.0	72.5
ycf3-trnS-GGA_p5	TTGGCTACAATCGAAGAGGTC	TTCCAATGCTACGCCTTGAA	47.6	45.0	57.8	57.8	568-574 bp	1.0	72.5