Hotspot

rpoC2-rps2

LSC · 30473-30756 · 284 bp

Back to Urochloa

Pi 0.0434

MarkerSeek score 69.80

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 22
pi: 0.0434
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	ACGCAAGGTCTTCCAAAAGT	ACCAAAATGAACTCCCGCTT	45.0	45.0	57.9	58.0	1094-1097 bp	1.0	81.9
rpoC2-rps2_p2	ACGCAAGGTCTTCCAAAAGT	TTTTCCCTGACTTGCTGCAT	45.0	45.0	57.9	58.0	1238-1241 bp	1.0	81.6
rpoC2-rps2_p3	AAACATCAGAAGCCCCGTTT	ACCAAAATGAACTCCCGCTT	45.0	45.0	58.0	58.0	388-391 bp	0.75	73.4
rpoC2-rps2_p4	AAACATCAGAAGCCCCGTTT	TTTTCCCTGACTTGCTGCAT	45.0	45.0	58.0	58.0	532-535 bp	0.75	72.5
rpoC2-rps2_p5	AAACATCAGAAGCCCCGTTT	GCGAGATCTGGGTCACAATT	45.0	50.0	58.0	58.0	924-927 bp	0.75	71.5