Hotspot

psbM-petN

LSC · 17558-18270 · 713 bp

Back to Urochloa

Pi 0.0321

MarkerSeek score 77.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 40
pi: 0.0321
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	AGTATGGGGGAGAAGTGGAC	40.0	55.0	56.7	58.1	736-762 bp	1.0	76.9
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.7	58.1	735-761 bp	1.0	76.9
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TATGGGGGAGAAGTGGACTC	40.0	55.0	56.7	57.8	734-760 bp	1.0	76.5
psbM-petN_p4	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	794-820 bp	1.0	76.4
psbM-petN_p5	AAAACAGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.7	58.2	731-757 bp	1.0	76.2