Hotspot

trnD-GUC-psbM

LSC · 16411-17452 · 1042 bp

Back to Urochloa

Pi 0.0226

MarkerSeek score 60.30

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 44
pi: 0.0226
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AGCTCGGCCCAAAAATCTAG	TCCGTGGATGCAGTGAAAAA	50.0	45.0	58.0	58.0	1783-1822 bp	1.0	84.3
trnD-GUC-psbM_p2	AATTCTTCCTGGGTCGATGC	TCCGTGGATGCAGTGAAAAA	50.0	45.0	58.0	58.0	1866-1905 bp	1.0	84.1
trnD-GUC-psbM_p3	AGCTCGGCCCAAAAATCTAG	CGCGTATTATTTTTCGCCCC	50.0	50.0	58.0	58.0	1804-1843 bp	0.75	72.6
trnD-GUC-psbM_p4	CTGTATATGGCGCTACCGTC	TCCGTGGATGCAGTGAAAAA	55.0	45.0	58.0	58.0	2052-2056 bp	0.5	59.8
trnD-GUC-psbM_p5	CTGTATATGGCGCTACCGTC	CGCGTATTATTTTTCGCCCC	55.0	50.0	58.0	58.0	2073-2077 bp	0.5	59.8