Hotspot

trnM-CAU-trnE-UUC

LSC · 15244-15764 · 521 bp

Back to Urochloa

Pi 0.0231

MarkerSeek score 65.30

Primer available yes

Label trnM-CAU-trnE-UUC

Feature Metadata

label_name: trnM-CAU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 22
pi: 0.0231
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnE-UUC_p1	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	632-635 bp	1.0	70.4
trnM-CAU-trnE-UUC_p2	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	611-614 bp	1.0	70.4
trnM-CAU-trnE-UUC_p3	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	595-598 bp	1.0	70.4
trnM-CAU-trnE-UUC_p4	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	574-577 bp	1.0	70.4
trnM-CAU-trnE-UUC_p5	TGGTAAGGCATAAGTCATCGG	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	600-603 bp	1.0	69.6