Hotspot

ndhG-ndhI

SSC · 118314-118694 · 381 bp

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Pi 0.0376
MarkerSeek score 55.10
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
381
variable_sites
34
pi
0.0376
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AACAATCCCGTACCATGACG AATCAAATAGCTCTGGGGCG 50.0 50.0 58.0 58.0 846 bp 1.0 84.6
ndhG-ndhI_p2 GGTTTGCCCTTGTAGTCCAA AATCAAATAGCTCTGGGGCG 50.0 50.0 58.0 58.0 865 bp 1.0 84.5
ndhG-ndhI_p3 GGTTTGCCCTTGTAGTCCAA AATCAGTACGAGCTGCAAGG 50.0 50.0 58.0 58.0 1236 bp 1.0 83.5
ndhG-ndhI_p4 AACAATCCCGTACCATGACG AATCAGTACGAGCTGCAAGG 50.0 50.0 58.0 58.0 1217 bp 1.0 83.5
ndhG-ndhI_p5 GCACCTATCAAACCGGCTAA AATCAGTACGAGCTGCAAGG 50.0 50.0 58.0 58.0 1354 bp 1.0 83.1