Hotspot

rpl32-trnL-UAG

SSC · 112804-113629 · 826 bp

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Pi 0.0413
MarkerSeek score 75.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
826
variable_sites
78
pi
0.0413
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 925-965 bp 1.0 88.4
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 926-966 bp 1.0 88.4
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT TAGAAAATGACATGCCGCCA 45.0 45.0 57.8 57.9 988-1028 bp 1.0 86.9
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT TCCGCATAAAAGAGCTGCAT 45.0 45.0 57.8 58.0 1547-1587 bp 1.0 86.2
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT GCTGCATAGCCCAATACCAT 45.0 50.0 57.8 58.1 1534-1574 bp 1.0 85.9